Detailed information of TA system TA184706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 41460..42067
• Replicon: plasmid p97_1_56kb
• Accession: NZ_CP066570
• Organism: Enterococcus faecium strain Dallas 97_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP88_RS15435
• Protein ID: WP_002321032.1
• Coordinates: 41460..41810 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP88_RS15440
• Protein ID: WP_002287514.1
• Coordinates: 41804..42067 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP88_RS15395	36760..37053	-	294	WP_002322251.1	hypothetical protein	-
JCP88_RS15400	37065..37244	-	180	WP_002322386.1	hypothetical protein	-
JCP88_RS15405	37241..37591	-	351	WP_000568378.1	hypothetical protein	-
JCP88_RS15410	37584..38909	-	1326	WP_196834128.1	Y-family DNA polymerase	-
JCP88_RS15415	39078..39662	-	585	WP_002299575.1	recombinase family protein	-
JCP88_RS15420	39873..40076	-	204	WP_002299573.1	hypothetical protein	-
JCP88_RS15425	40086..40508	-	423	WP_002288787.1	hypothetical protein	-
JCP88_RS15430	40822..41436	+	615	WP_002318230.1	recombinase family protein	-
JCP88_RS15435	41460..41810	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP88_RS15440	41804..42067	-	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP88_RS15445	42305..43450	-	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP88_RS15450	43462..43863	-	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP88_RS15455	44030..44302	-	273	WP_002348912.1	hypothetical protein	-
JCP88_RS15460	44284..44700	-	417	WP_002305764.1	hypothetical protein	-
JCP88_RS15465	44716..44967	-	252	WP_002305766.1	hypothetical protein	-
JCP88_RS15470	44967..45173	-	207	WP_002296239.1	hypothetical protein	-
JCP88_RS15475	45618..45878	-	261	WP_002321303.1	hypothetical protein	-
JCP88_RS15480	46244..46717	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56380	56380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184706 WP_002321032.1 NZ_CP066570:c41810-41460 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184706 NZ_CP087784:1777944-1778222 [Moraxella bovis]
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184706 WP_002287514.1 NZ_CP066570:c42067-41804 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184706 NZ_CP087784:1778209-1778496 [Moraxella bovis]
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0