Detailed information of TA system TA184656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2907055..2907638
• Replicon: chromosome
• Accession: NZ_CP066552
• Organism: Pectobacterium carotovorum strain 2A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JFY74_RS12840
• Protein ID: WP_198996892.1
• Coordinates: 2907306..2907638 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7T5U8Q8
• Locus tag: JFY74_RS12835
• Protein ID: WP_038925224.1
• Coordinates: 2907055..2907306 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY74_RS12810	2904802..2905407	+	606	WP_198996887.1	type I restriction-modification system subunit M	-
JFY74_RS12815	2905476..2905736	+	261	WP_198996888.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JFY74_RS12820	2905807..2906178	+	372	WP_198996889.1	helix-turn-helix domain-containing protein	-
JFY74_RS12825	2906372..2906596	-	225	WP_198996890.1	hypothetical protein	-
JFY74_RS12830	2906633..2906923	+	291	WP_198996891.1	transposase	-
JFY74_RS12835	2907055..2907306	+	252	WP_038925224.1	PbsX family transcriptional regulator	Antitoxin
JFY74_RS12840	2907306..2907638	+	333	WP_198996892.1	endoribonuclease MazF	Toxin
JFY74_RS12850	2908406..2909065	+	660	WP_103860384.1	FtsH protease modulator YccA	-
JFY74_RS12855	2909175..2909504	+	330	WP_198996893.1	sulfurtransferase TusE	-
JFY74_RS12860	2909517..2909795	-	279	WP_198996894.1	acylphosphatase	-
JFY74_RS12865	2910018..2910326	+	309	WP_198996895.1	heat shock protein HspQ	-
JFY74_RS12870	2910423..2910836	-	414	WP_198996896.1	CoA-binding protein	-
JFY74_RS12875	2911030..2911554	+	525	WP_198996897.1	DUF2057 family protein	-
JFY74_RS12880	2911692..2912150	+	459	WP_010276040.1	methylglyoxal synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• Antitoxin PemI-like (3-82)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12176.95 Da
• Isoelectric Point: 6.9010

>T184656 WP_198996892.1 NZ_CP066552:2907306-2907638 [Pectobacterium carotovorum]
MVSRFVPDSGDLIWLDFDPVVGYEQGGHRPAVVLSPFPYNNLTGMCLCTPCTTQRKGYNFEVELSGDRESVALADQVTCV
DWRNRKATKKGKVTADELELIRAKAKALIG

Nucleotide

• Length: 333 bp

>T184656 NZ_CP087765:c356886-356608 [Moraxella bovis]
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9466.97 Da
• Isoelectric Point: 4.8047

>AT184656 WP_038925224.1 NZ_CP066552:2907055-2907306 [Pectobacterium carotovorum]
MIDVPVKKWGNSPAIRLSSSVMQAFDMTFNDSFDMEIRETEIALIPKKKPKYQFTLDELLEGMTPEHVHEKVDFGAPVGK
ELL

Nucleotide

• Length: 252 bp

>AT184656 NZ_CP087765:c356621-356334 [Moraxella bovis]
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA

Structures

Antitoxin

• AlphaFold DB: A0A7T5U8Q8