Detailed information of TA system TA184653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 976804..977444
• Replicon: chromosome
• Accession: NZ_CP066552
• Organism: Pectobacterium carotovorum strain 2A

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JFY74_RS04270
• Protein ID: WP_180790514.1
• Coordinates: 977028..977444 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6D0W2
• Locus tag: JFY74_RS04265
• Protein ID: WP_011095187.1
• Coordinates: 976804..977031 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY74_RS04245	971956..972459	-	504	WP_039507077.1	M48 family metallopeptidase	-
JFY74_RS04250	972628..973821	-	1194	WP_198998252.1	aspartate/tyrosine/aromatic aminotransferase	-
JFY74_RS04255	973885..974499	-	615	WP_198998253.1	YitT family protein	-
JFY74_RS04260	974751..975230	-	480	WP_010295086.1	Lrp/AsnC family transcriptional regulator	-
JFY74_RS04265	976804..977031	+	228	WP_011095187.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JFY74_RS04270	977028..977444	+	417	WP_180790514.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFY74_RS04275	977583..978221	-	639	WP_198998254.1	hypothetical protein	-
JFY74_RS04280	978214..979296	-	1083	WP_198998255.1	alanine racemase	-
JFY74_RS04285	979338..980744	-	1407	WP_010295074.1	replicative DNA helicase	-
JFY74_RS04290	981066..982049	+	984	WP_198998256.1	quinone oxidoreductase	-
JFY74_RS04295	982135..982374	+	240	WP_198998257.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	lpxC	833496..1047599	214103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15190.68 Da
• Isoelectric Point: 8.7720

>T184653 WP_180790514.1 NZ_CP066552:977028-977444 [Pectobacterium carotovorum]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSASTYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIALAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Nucleotide

• Length: 417 bp

>T184653 NZ_CP087765:37061-37462 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTACATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCGTACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8894.05 Da
• Isoelectric Point: 4.5216

>AT184653 WP_011095187.1 NZ_CP066552:976804-977031 [Pectobacterium carotovorum]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEERADADFMTERQDVVEEGRFEL

Nucleotide

• Length: 228 bp

>AT184653 NZ_CP087765:36786-37046 [Moraxella bovis]
ATGTCATATACACTTATGGGAGAATGCCATGCAAACCAGTGTTTTTTTAATAATCGCACCCAAGCGGTTCGCATTCCTGC
CAAATTTCGCTTTGATGACGATGTCAAAGCCGTCATGATTAGAAAAGTGGGCAATGAGCGGATTTTGACCCCAATTGATG
GTGTGTGGGACAGCTTTTTTTTGAGTGATGAGCGAGCGAGTGATGATTTTATGGCAAACCGCCCGACCATGCCAAAAAGC
GAGCGATTGACTTTTGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J5XN10