Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 59780..60381 Replicon plasmid p2
Accession NZ_CP066549
Organism Lactiplantibacillus plantarum strain SHY 21-2

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag JFT18_RS15280 Protein ID WP_001748110.1
Coordinates 59780..60124 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag JFT18_RS15285 Protein ID WP_003643337.1
Coordinates 60118..60381 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFT18_RS15265 55352..56151 + 800 WP_088899809.1 IS5 family transposase -
JFT18_RS15275 58201..59460 + 1260 WP_087511045.1 cytochrome P450 -
JFT18_RS15280 59780..60124 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JFT18_RS15285 60118..60381 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JFT18_RS15290 60467..61054 + 588 WP_087511044.1 site-specific integrase -
JFT18_RS15295 61250..61501 + 252 WP_002816285.1 IS3 family transposase -
JFT18_RS15300 61555..62424 + 870 WP_143443855.1 IS3 family transposase -
JFT18_RS15310 63675..64670 + 996 WP_087510881.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..68317 68317
- inside IScluster/Tn - - 43986..61501 17515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T184647 WP_001748110.1 NZ_CP066549:c60124-59780 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T184647 NZ_CP087718:24594-24950 [Acinetobacter pittii]
ATGTGGATAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTAGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCTTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCAGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT184647 WP_003643337.1 NZ_CP066549:c60381-60118 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT184647 NZ_CP087718:24943-25245 [Acinetobacter pittii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATCTCTCAAAAGGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGCGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References