Detailed information of TA system TA184645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3235077..3235662
• Replicon: chromosome
• Accession: NZ_CP066546
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7S5HMS4
• Locus tag: JE032_RS14815
• Protein ID: WP_016705075.1
• Coordinates: 3235369..3235662 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JE032_RS14810
• Protein ID: WP_053268634.1
• Coordinates: 3235077..3235367 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE032_RS14800	3232589..3233971	+	1383	WP_009048175.1	efflux transporter outer membrane subunit	-
JE032_RS14805	3234090..3234677	+	588	WP_009048176.1	hypothetical protein	-
JE032_RS14810	3235077..3235367	-	291	WP_053268634.1	putative addiction module antidote protein	Antitoxin
JE032_RS14815	3235369..3235662	-	294	WP_016705075.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JE032_RS14820	3235897..3237291	-	1395	WP_053268635.1	VOC family protein	-
JE032_RS14825	3237556..3238680	+	1125	WP_053268636.1	diguanylate cyclase	-
JE032_RS14830	3238774..3240018	+	1245	WP_053268637.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (13-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10699.27 Da
• Isoelectric Point: 10.0959

>T184645 WP_016705075.1 NZ_CP066546:c3235662-3235369 [Pseudomonas chlororaphis subsp. aureofaciens]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T184645 NZ_CP087718:2891-3172 [Acinetobacter pittii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10295.84 Da
• Isoelectric Point: 8.0096

>AT184645 WP_053268634.1 NZ_CP066546:c3235367-3235077 [Pseudomonas chlororaphis subsp. aureofaciens]
MSDTLVPFDISTLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Nucleotide

• Length: 291 bp

>AT184645 NZ_CP087718:2588-2890 [Acinetobacter pittii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A7S5HMS4