Detailed information of TA system TA184644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3201114..3201718
• Replicon: chromosome
• Accession: NZ_CP066546
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JE032_RS14665
• Protein ID: WP_041988418.1
• Coordinates: 3201551..3201718 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JE032_RS14660
• Protein ID: WP_103330829.1
• Coordinates: 3201114..3201515 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE032_RS14645	3196770..3198659	+	1890	WP_198920004.1	PAS domain S-box protein	-
JE032_RS14650	3198656..3199291	+	636	WP_023968669.1	response regulator transcription factor	-
JE032_RS14655	3199433..3200149	+	717	WP_039968439.1	hypothetical protein	-
JE032_RS14660	3201114..3201515	-	402	WP_103330829.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JE032_RS14665	3201551..3201718	-	168	WP_041988418.1	type II toxin-antitoxin system HicA family toxin	Toxin
JE032_RS14670	3201984..3202487	-	504	WP_198920070.1	zinc ribbon domain-containing protein	-
JE032_RS14675	3203381..3203677	-	297	WP_198920005.1	GNAT family N-acetyltransferase	-
JE032_RS14680	3203839..3204837	-	999	WP_198920006.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-54)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6219.35 Da
• Isoelectric Point: 10.6778

>T184644 WP_041988418.1 NZ_CP066546:c3201718-3201551 [Pseudomonas chlororaphis subsp. aureofaciens]
MIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 168 bp

>T184644 NZ_CP087718:2588-2890 [Acinetobacter pittii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14565.51 Da
• Isoelectric Point: 4.5918

>AT184644 WP_103330829.1 NZ_CP066546:c3201515-3201114 [Pseudomonas chlororaphis subsp. aureofaciens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELARDGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT184644 NZ_CP087718:2891-3172 [Acinetobacter pittii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA