Detailed information of TA system TA184640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 132703..133292
• Replicon: chromosome
• Accession: NZ_CP066546
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain SPS-41

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JE032_RS00645
• Protein ID: WP_028683468.1
• Coordinates: 132993..133292 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JE032_RS00640
• Protein ID: WP_007924096.1
• Coordinates: 132703..132996 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE032_RS00620	128244..129407	-	1164	WP_041987444.1	acyltransferase	-
JE032_RS00625	129602..130792	-	1191	WP_028683472.1	serine hydrolase	-
JE032_RS00630	130919..131806	+	888	WP_009051074.1	LysR family transcriptional regulator	-
JE032_RS00635	132178..132570	+	393	WP_198919430.1	hypothetical protein	-
JE032_RS00640	132703..132996	-	294	WP_007924096.1	putative addiction module antidote protein	Antitoxin
JE032_RS00645	132993..133292	-	300	WP_028683468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JE032_RS00650	133463..133912	+	450	WP_009051078.1	DUF3828 domain-containing protein	-
JE032_RS00655	133937..134947	-	1011	WP_198920052.1	NAD(P)-dependent alcohol dehydrogenase	-
JE032_RS00660	135005..135304	-	300	WP_009051080.1	ArsR family transcriptional regulator	-
JE032_RS00665	135368..136459	-	1092	WP_009051081.1	nitrite reductase, copper-containing	-
JE032_RS00670	136720..136962	-	243	WP_009051082.1	hypothetical protein	-
JE032_RS00675	137448..137690	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11256.97 Da
• Isoelectric Point: 10.4277

>T184640 WP_028683468.1 NZ_CP066546:c133292-132993 [Pseudomonas chlororaphis subsp. aureofaciens]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQSRDIELAKYLARRWSRS

Nucleotide

• Length: 300 bp

>T184640 NZ_CP087711:2935795-2936292 [Plesiomonas shigelloides]
ATGATTATTTCTAAACCTGAGGTCTTGAGTAAAGAACACCGTGTTGAGAGTTTTGATTGCGGAAATCTCTCATTAAATGA
ATGGCTGATCCGTCATTCTAGGCAGGCACAGTCTAGTGGATCAGCCAAAACCTTTGTTGTAACCGACAGTGGTAGTGTTG
TCGGCTATTTTAGCCTGACAGTAGGTCAAGTAGATACCTATGAGGTTCCTGAACGTGTACGTCAAGGAATGGGGCAGTAC
CCAATACCGGTGGTAATACTTGCTCGATTAGCCGTATCTATTAAATATCAAAAAATGGGTATTGGTGTTGGGCTACTTCA
GGATGCAATTAAAAGAACAGTCTTGATTGCAGAACAAGTTGGTATTCGTGCACTTCTTACTCATCCGATTGATAATGCTG
CTGCACAATTTTACGAACAATTCGGATTTGTTGCGTCGCCAGTGCGTGAAAAACAGTTGGTATTATTACTGAAAGATGCC
CGAAAGCTATTGCGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10430.94 Da
• Isoelectric Point: 5.6558

>AT184640 WP_007924096.1 NZ_CP066546:c132996-132703 [Pseudomonas chlororaphis subsp. aureofaciens]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT184640 NZ_CP087711:2935496-2935795 [Plesiomonas shigelloides]
ATGCCTAATTGTGCATCAGTGCCAGCTTCGCGCTCTGTTCGTACAGCTCGTCTGGGCTTGCGTGCAACACCTGAGCAAGA
GGCTGTTTTACGGCGAGCAGCGGATATCGCACATAAGTCATTGACTGACTTCATTTTGGATAGTGCGTGTCACGCGGCTG
AACAAGCGTTATTAGATCAACGTCTATTTGTCACATCTGGTGAACAGTATAAGACCTTCTTGGATATGCTCGATCAGCCA
GAAAAGGAAAATGAAGGTCTGAACGATTTGTTTGCTCGTAAAGCTCCTTGGGATAAGTAA