184639

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 76246..76889 Replicon plasmid pBTS_02
Accession NZ_CP066544
Organism Klebsiella pneumoniae strain BTS

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag D6T75_RS27765 Protein ID WP_001044770.1
Coordinates 76473..76889 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag D6T75_RS27760 Protein ID WP_001261282.1
Coordinates 76246..76476 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T75_RS27725 71469..72248 - 780 WP_013214009.1 site-specific integrase -
D6T75_RS27730 72432..73436 - 1005 WP_200303248.1 hypothetical protein -
D6T75_RS27735 73466..73669 - 204 WP_011977813.1 hypothetical protein -
D6T75_RS27740 73715..74236 - 522 WP_013214008.1 hypothetical protein -
D6T75_RS27745 74294..74686 - 393 WP_011977811.1 hypothetical protein -
D6T75_RS27750 74723..75667 - 945 WP_011977810.1 hypothetical protein -
D6T75_RS27755 75828..76289 - 462 WP_014343465.1 hypothetical protein -
D6T75_RS27760 76246..76476 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D6T75_RS27765 76473..76889 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
D6T75_RS27770 76963..78525 + 1563 WP_004206609.1 AAA family ATPase -
D6T75_RS27775 78510..79532 + 1023 WP_000361404.1 helicase UvrD -
D6T75_RS27780 79788..80485 + 698 Protein_106 IS1 family transposase -
D6T75_RS27785 80514..80786 + 273 Protein_107 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaCTX-M-65 - 1..88050 88050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T184639 WP_001044770.1 NZ_CP066544:76473-76889 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T184639 NZ_CP087711:2934029-2934298 [Plesiomonas shigelloides]
ATGAGTGAAAGGTTATTGTGTTGGACTGATGAGTCTTGGGATGACTATATTTACTGGCAATCTCAGGATAAGAAAACTTT
GAAAAGAATCAATAAGTTAATTATTGATGTTAAACGCACGCCGTTTGAAGGCATCGGAAAGCCTGAAGCCTTGAGAGAAA
ACCTGTCAGGATTCTGGTCTCGCCGCATAGATGATACAAACCGTTTAGTCTATGCGGTTGATGACCTATCAATTACCATC
ATTTCTTGTCGTTATCACTATCAAGCGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT184639 WP_001261282.1 NZ_CP066544:76246-76476 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT184639 NZ_CP087711:2933782-2934036 [Plesiomonas shigelloides]
ATGAGAATCGTATCCTTTACTGAAGCTCGTAACAGCTTGAAATCAGTACTTGATTCAGTTGTAAACGATGCAGATACAAC
CATCATTACTCGCCGTGATGCGGATGATGCAGTTGTGATGTCTCTAGATTATTACAATAGCCTGATGGAAACCGTTCACT
TGTTGCGATCCCCCGCGAACGCAGAGCATTTGAACCGTTCTATAGCTCAATTTAAAGCTGGTCGAGCCACGCAGCGAGAG
TTGAAGGATGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References