Detailed information of TA system TA184620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 986248..986867
• Replicon: chromosome
• Accession: NZ_CP066543
• Organism: Klebsiella pneumoniae strain BTS

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: D6T75_RS05850
• Protein ID: WP_200302835.1
• Coordinates: 986248..986466 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J2DPF6
• Locus tag: D6T75_RS05855
• Protein ID: WP_002892066.1
• Coordinates: 986493..986867 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T75_RS05815	982295..982558	+	264	WP_002892011.1	type B 50S ribosomal protein L31	-
D6T75_RS05820	982558..982698	+	141	WP_002892018.1	type B 50S ribosomal protein L36	-
D6T75_RS05825	982695..983393	-	699	WP_002892021.1	GNAT family N-acetyltransferase	-
D6T75_RS05830	983494..984945	+	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
D6T75_RS05835	984920..985390	-	471	WP_002892026.1	YlaC family protein	-
D6T75_RS05840	985411..985551	+	141	WP_004147370.1	hypothetical protein	-
D6T75_RS05845	985523..986089	-	567	WP_002892030.1	maltose O-acetyltransferase	-
D6T75_RS05850	986248..986466	-	219	WP_200302835.1	hemolysin expression modulator Hha	Toxin
D6T75_RS05855	986493..986867	-	375	WP_002892066.1	Hha toxicity modulator TomB	Antitoxin
D6T75_RS05860	987353..990499	-	3147	WP_020326861.1	multidrug efflux RND transporter permease subunit	-
D6T75_RS05865	990522..991715	-	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8642.07 Da
• Isoelectric Point: 9.4641

>T184620 WP_200302835.1 NZ_CP066543:c986466-986248 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKKKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T184620 NZ_CP087705:c498648-498370 [Halopseudomonas aestusnigri]
ATGATCACAGGATTCCAACATAAAGGGCTAGAAGCCTTCTACAAGACTGGAACGACCCGAGGCATTCAAGCGGCACATGC
CGCCAAACTAAGTCGCATCCTGGCGTTACTGGACGTTGCCGCTGGCCCAGACGACCTCAACATTCCTTCATTCAAGTTAC
ACCAGTTGAAAGGTGAGCTGAACGGGTACTGGTCAATTAGGGTAAACGGCAACTGGCGTGTGACCTTTCGGTTCATTGGA
GTGGATGTCGAATTGGTCAATTACGTCGATTACCACTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT184620 WP_002892066.1 NZ_CP066543:c986867-986493 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT184620 NZ_CP087705:c498357-498073 [Halopseudomonas aestusnigri]
ATGATGATGAACCCTCCGCACCCCGGCGAACTGCTGCGGGAAGACGTCATTGCTGAGCTGGGCCTGTCCGTCAAGGAAAC
GGCTGAGCGACTAGGCATGTCCCGTGTAGCTCTGTCCCGGGTACTGAACGGCCGAGCAGCGATCAGCCCTGACCTTGCGC
TTCGCTTAGAAATGGCAGGCGTGAGCACAGCCCGAGCCTGGCTCGCCATGCAGGTGAACTATGATCTGGCCCAAGCCAGG
CAACACCCGCAACCACCGATCAAGGCACTGCAACCAGTTGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GJ93