Detailed information of TA system TA184607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53680..54416
• Replicon: plasmid pCTXM15_090374
• Accession: NZ_CP066535
• Organism: Klebsiella pneumoniae strain WCHKP090374

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: H3G96_RS27165
• Protein ID: WP_003026803.1
• Coordinates: 53934..54416 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: H3G96_RS27160
• Protein ID: WP_003026799.1
• Coordinates: 53680..53946 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G96_RS27115	49742..50104	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
H3G96_RS27120	50154..50504	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
H3G96_RS27125	50862..51131	+	270	WP_004152102.1	hypothetical protein	-
H3G96_RS27130	51119..51694	+	576	WP_004152103.1	hypothetical protein	-
H3G96_RS27135	51725..52219	+	495	WP_004152104.1	DNA-binding protein	-
H3G96_RS27140	52263..52631	+	369	WP_004152105.1	hypothetical protein	-
H3G96_RS27145	52665..52868	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
H3G96_RS27150	52917..53174	+	258	WP_004152107.1	hypothetical protein	-
H3G96_RS27155	53250..53504	+	255	WP_004152108.1	hypothetical protein	-
H3G96_RS27160	53680..53946	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
H3G96_RS27165	53934..54416	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
H3G96_RS27170	54624..55970	+	1347	WP_077253535.1	ISNCY family transposase	-
H3G96_RS27175	56019..56414	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
H3G96_RS27180	56562..57816	-	1255	Protein_58	IS3 family transposase	-
H3G96_RS27185	57904..58866	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1 / tet(A) / qnrB1	-	1..192589	192589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T184607 WP_003026803.1 NZ_CP066535:53934-54416 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T184607 NZ_CP087695:c1361355-1361032 [Xylella taiwanensis]
ATGACTCAACGCGGCGACCTTGTAACGGTTTCCCTGCAAGGCGATTATGGCAAACCTAGACCGGCGTTAATCCTCCAATC
AGACCTGCTGATGGAGCTAGATAGTGTCGTACTGTGTCCGGTCACAAGTGAGCTACGTAATGCGATATTTCGCATCACCG
TTGAACCAAACATAGCCAATGGGCTACGGACGCTCTCGCAAATCATGGTAGACAAAATTTCAACACTGCCGCGTAGCAAA
ATCAGCAAACCTTTTGGACATCTCAATGACGAGAGGATAAAAGCAGTAGAAAAAGCACTGTTGCTCATCGTTGGTATCAT
CTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT184607 WP_003026799.1 NZ_CP066535:53680-53946 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT184607 NZ_CP087695:c1361573-1361352 [Xylella taiwanensis]
ATGGCATTCATTCACGAACCGACTAGCAAAGTAGCTCGCCACCGAAAGCAAATGCGCGCAGCGGGATTACGTCCTGTCCA
GTTCTGGGTACCGGATACGCGCACACCGGAATTCGCCGCTGAAATTCGGAGCCAGTGCAGGAGCCTGAAGAACGACCCGG
AAGAAGCTGACGCTTTGCGCTTCACCGAAGAAGCCATAACCCATATTGAAGGCTGGAAATGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66