Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5386549..5387174 Replicon chromosome
Accession NZ_CP066534
Organism Klebsiella pneumoniae strain WCHKP090374

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H3G96_RS26525 Protein ID WP_002882817.1
Coordinates 5386549..5386932 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H3G96_RS26530 Protein ID WP_004150355.1
Coordinates 5386932..5387174 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G96_RS26510 5383915..5384817 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H3G96_RS26515 5384814..5385449 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H3G96_RS26520 5385446..5386375 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H3G96_RS26525 5386549..5386932 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H3G96_RS26530 5386932..5387174 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H3G96_RS26535 5387379..5388296 + 918 WP_002882812.1 alpha/beta hydrolase -
H3G96_RS26540 5388310..5389251 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H3G96_RS26545 5389296..5389733 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H3G96_RS26550 5389730..5390590 - 861 WP_002882807.1 virulence factor BrkB family protein -
H3G96_RS26555 5390584..5391183 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T184606 WP_002882817.1 NZ_CP066534:c5386932-5386549 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T184606 NZ_CP087694:c2665487-2665233 [Xylella taiwanensis]
ATGAAGCGTAAGCACGCTAAGACCCTCAGCACCATAGACACGCGCCCTGTTTCCGCCAACATCCAATGGCGCGATATTGA
AGCACTCTTCTTGGAGCTTGGAGCGACAGTAGAAGAACGTGAAGGGTCTCGTGTGCTGGTGCGGCTGTTCGGTGAACGCC
GTGTATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGCGCTGTTGAATCGATCCGAGCTTGGTTGAAATCGAAC
GGAGTGACACCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT184606 WP_004150355.1 NZ_CP066534:c5387174-5386932 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT184606 NZ_CP087694:c2665236-2664901 [Xylella taiwanensis]
ATGAAAAACATCATGACCATTGAAGGCTTCAAAGCCATCATTACCTATGATCCGGATATCGACATGTTTCGCGGTGAGTT
TGTCGGTATCAATGGCGGTGCTGATTTCTATGCCAAAGACCTCAAGGGCCTTCGTCGAGAAGGGGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCGAAGAAGATGACGTCGAACCACGCAAATCCTACTCAGGGAAGTTCAATGCAAGGATCTCTCCT
GAACTCCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGAATCAGTTTGAACCAATTTGTAGAACAGGCGATTCAGCA
TGAAATCCACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References