Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5303590..5304215 Replicon chromosome
Accession NZ_CP066528
Organism Klebsiella pneumoniae strain WCHKP090361

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H3G66_RS26090 Protein ID WP_002882817.1
Coordinates 5303590..5303973 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H3G66_RS26095 Protein ID WP_004150355.1
Coordinates 5303973..5304215 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G66_RS26075 5300956..5301858 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H3G66_RS26080 5301855..5302490 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H3G66_RS26085 5302487..5303416 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H3G66_RS26090 5303590..5303973 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H3G66_RS26095 5303973..5304215 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H3G66_RS26100 5304420..5305337 + 918 WP_002882812.1 alpha/beta hydrolase -
H3G66_RS26105 5305351..5306292 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H3G66_RS26110 5306337..5306774 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H3G66_RS26115 5306771..5307631 - 861 WP_002882807.1 virulence factor BrkB family protein -
H3G66_RS26120 5307625..5308224 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T184576 WP_002882817.1 NZ_CP066528:c5303973-5303590 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T184576 NZ_CP087689:2823816-2824232 [Xylella taiwanensis]
TTGAGCGGATATTTGCTCGATACGAACATCATTAGCGACATGATCCGTAATCCGTTTGGACTAGTTGCGTGTCGCATTGA
AAAGGTCGATCCAAAAGAGGTTTGCACTAGCATCGTTGTCGCTGCTGAGTTGCGGTATGGTTGTGCAAAGAAAGGATCGA
CCAAGTTGCTTGCGAAGGTGAAGCTCATGCTTGAAACAATTCCTGTGCTGCCTTTGGATATTCCTACCGACGCTGAATAT
GGTCGCATTCGTGCAGAGTTGGAGTCAGTAGGACAAACTATCGGCTTAAATGATCTATTGATAGGTACTCATGCTTATGT
ACTGGGGATGACATTGGTAACGGATAATGTTAAAGAGTTTAAGCGTATTCGTGGCCTTAAGATCGAAAATTGGCTGGAAT
GTGCATCCTCGGTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT184576 WP_004150355.1 NZ_CP066528:c5304215-5303973 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT184576 NZ_CP087689:2823547-2823819 [Xylella taiwanensis]
ATGTCTATTTCTATGCCATCATCTAGACTCAAGGAAGCTAAGCTGTTCCGCAACAATCGCAGTCAGGCCGTGAGAATTCC
GGTTGAGTTTGAATTACCGGGGGATCGAGTATTGATTCATCGCGAGGGTAGCAAGCTCATCATTGAGCCTGTGAGCATGC
CTACCAACATTATAGAATTGTTAGCGCAGTGGAAAACGGAAGATCCGCTTGGACTTGAAGATCAGTTTCCTGAAATTGAG
GACATGCTTGCTAAGCCGGATGACATATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References