Detailed information of TA system TA184558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 184083..184726
• Replicon: plasmid p1_090357
• Accession: NZ_CP066525
• Organism: Klebsiella pneumoniae strain WCHKP090357

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H3G71_RS28800
• Protein ID: WP_014386165.1
• Coordinates: 184310..184726 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: H3G71_RS28795
• Protein ID: WP_032408893.1
• Coordinates: 184083..184313 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G71_RS28770	179156..180151	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
H3G71_RS28775	180158..181306	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
H3G71_RS28780	181477..182634	-	1158	WP_032408895.1	acetylornithine deacetylase	-
H3G71_RS28785	182650..183324	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
H3G71_RS28790	183329..183763	-	435	WP_000103648.1	RidA family protein	-
H3G71_RS28795	184083..184313	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H3G71_RS28800	184310..184726	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
H3G71_RS28805	185049..186017	+	969	WP_074422984.1	IS5 family transposase	-
H3G71_RS28810	186197..186571	-	375	WP_032408891.1	hypothetical protein	-
H3G71_RS28815	186627..186953	-	327	WP_013023807.1	hypothetical protein	-
H3G71_RS28820	186950..187678	-	729	WP_001568058.1	plasmid SOS inhibition protein A	-
H3G71_RS28825	187675..188106	-	432	WP_001568057.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..206852	206852
	-	flank	IS/Tn	-	-	185049..186017	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T184558 WP_014386165.1 NZ_CP066525:184310-184726 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T184558 NZ_CP087686:c577-305 [Xylella taiwanensis]
ATGCGCCGTCCTGCTTATTCTTCGCAGTTCAAGCGTGATGTGCGAGTGTCTCAGAAGCGCGGTAAGGACATAGAGAAACT
TAAGATTCTAATAACTTTGCTGATTGAGGGTAGTCCGTTGCCTGCGATGTATTTGGATCATCCGCTTAAGGGGCAGTGGC
GTAGTTTCCGGGATGCTCATGTTGAGCCGGATTGGTTGTTAATCTATAAGGTTGATGGTGATGTGGTGCGCTTTGAGCGT
ACTGGTCGTCATGCTGATTTGTTCGACGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT184558 WP_032408893.1 NZ_CP066525:184083-184313 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT184558 NZ_CP087686:c837-577 [Xylella taiwanensis]
ATGGCTGCAAATGCACTTGTGCGTGCCAGAGTTGATGAGGCTCTTAAAGATGAGGCCGCTTCTGTACTTGCGACGATGGG
CCTTACCGTATCTGATCTTATACGTATCACCTTGACTAAAGTGGCTCGTGAAAAAATGTTGCCTTTTGAGATGCACTCAC
CTAATAGGCTTACGGTTGAGACTTTTGTTAAAAGTGAGTGTGGTGAGGATGTGCATTATGCTGAAAGTGCTGATGCCATG
TTTAAAGATCTTGGTATCTAA