Detailed information of TA system TA184557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 160187..160932
• Replicon: plasmid p1_090357
• Accession: NZ_CP066525
• Organism: Klebsiella pneumoniae strain WCHKP090357

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: H3G71_RS28670
• Protein ID: WP_032408901.1
• Coordinates: 160441..160932 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: H3G71_RS28665
• Protein ID: WP_014386183.1
• Coordinates: 160187..160453 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G71_RS28615	155739..156152	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
H3G71_RS28620	156153..156431	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
H3G71_RS28625	156421..156741	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H3G71_RS28630	156822..157046	-	225	WP_014386189.1	hypothetical protein	-
H3G71_RS28635	157057..157269	-	213	WP_014386188.1	hypothetical protein	-
H3G71_RS28640	157331..157657	-	327	WP_014386187.1	hypothetical protein	-
H3G71_RS28645	158294..158644	-	351	WP_014386186.1	hypothetical protein	-
H3G71_RS28650	158641..158913	-	273	WP_032408902.1	hypothetical protein	-
H3G71_RS28655	159103..159588	+	486	WP_014386185.1	hypothetical protein	-
H3G71_RS28660	159832..159990	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
H3G71_RS28665	160187..160453	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
H3G71_RS28670	160441..160932	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
H3G71_RS28675	161373..161624	-	252	WP_032408900.1	aldo/keto reductase	-
H3G71_RS28680	161821..163413	-	1593	Protein_177	IS66 family transposase	-
H3G71_RS28685	163444..163794	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
H3G71_RS28690	163791..164231	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
H3G71_RS28695	164493..165248	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..206852	206852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T184557 WP_032408901.1 NZ_CP066525:160441-160932 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T184557 NZ_CP087685:2793244-2793648 [Xylella taiwanensis]
TTGCTCGATACGAACATCATTAGCGACATGATCCGTAATCCGTTTGGACTAGTTGCGTGTCGCATTGAAAAGGTCGATCC
AAAAGAGGTTTGCACTAGCATCGTTGTCGCTGCTGAGTTGCGGTATGGTTGTGCAAAGAAAGGATCGACCAAGTTGCTTG
CGAAGGTGAAGCTCATGCTTGAAACAATTCCTGTGCTGCCTTTGGATATTCCTACCGACGCTGAATATGGTCGCATTCGT
GCAGAGTTGGAGTCAGTAGGACAAACTATCGGCTTAAATGATCTATTGATAGGTACTCATGCTTATGTACTGGGGATGAC
ATTGGTAACGGATAATGTTAAAGAGTTTAAGCGTATTCGTGGCCTTAAGATCGAAAATTGGCTGGAATGTGCATCCTCGG
TTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT184557 WP_014386183.1 NZ_CP066525:160187-160453 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT184557 NZ_CP087685:2792963-2793235 [Xylella taiwanensis]
ATGTCTATTTCTATGCCATCATCTAGACTCAAGGAAGCTAAGCTGTTCCGCAACAATCGCAGTCAGGCCGTGAGAATTCC
GGTTGAGTTTGAATTACCGGGGGATCGAGTATTGATTCATCGCGAGGGTAGCAAGCTCATCATTGAGCCTGTGAGCATGC
CTACCAACATTATAGAATTGTTAGCGCAGTGGAAAACGGAAGATCCGCTTGGACTTGAAGATCAGTTTCCTGAAATTGAG
GACATGCTTGCTAAGCCGGATGACATATTTTGA

Structures

Toxin

• AlphaFold DB: A0A331KSM6