Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 92999..93642 Replicon plasmid pKPC2_090357
Accession NZ_CP066524
Organism Klebsiella pneumoniae strain WCHKP090357

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H3G71_RS27405 Protein ID WP_001044770.1
Coordinates 92999..93415 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H3G71_RS27410 Protein ID WP_001261282.1
Coordinates 93412..93642 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G71_RS27380 88083..88442 + 360 WP_015493069.1 hypothetical protein -
H3G71_RS27385 89068..89442 + 375 WP_008324180.1 hypothetical protein -
H3G71_RS27390 89568..90272 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H3G71_RS27395 90356..91378 - 1023 WP_000361404.1 helicase UvrD -
H3G71_RS27400 91363..92925 - 1563 WP_004206609.1 AAA family ATPase -
H3G71_RS27405 92999..93415 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H3G71_RS27410 93412..93642 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H3G71_RS27415 93599..94060 + 462 WP_014343465.1 hypothetical protein -
H3G71_RS27420 94221..95165 + 945 WP_011977810.1 hypothetical protein -
H3G71_RS27425 95202..95594 + 393 WP_011977811.1 hypothetical protein -
H3G71_RS27430 95652..96173 + 522 WP_013214008.1 hypothetical protein -
H3G71_RS27435 96219..96422 + 204 WP_011977813.1 hypothetical protein -
H3G71_RS27440 96452..97456 + 1005 WP_011977814.1 hypothetical protein -
H3G71_RS27445 97640..98419 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 - 1..149116 149116
- flank IS/Tn - - 89568..90272 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T184552 WP_001044770.1 NZ_CP066524:c93415-92999 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T184552 NZ_CP087685:c616-344 [Xylella taiwanensis]
ATGCGCCGTCCTGCTTATTCTTCGCAGTTCAAGCGTGATGTGCGAGTGTCTCAGAAGCGCGGTAAGGACATAGAGAAACT
TAAGATTCTAATAACTTTGCTGATTGAGGGTAGTCCGTTGCCTGCGATGTATTTGGATCATCCGCTTAAGGGGCAGTGGC
GTAGTTTCCGGGATGCTCATGTTGAGCCGGATTGGTTGTTAATCTATAAGGTTGATGGTGATGTGGTGCGCTTTGAGCGT
ACTGGTCGTCATGCTGATTTGTTCGACGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT184552 WP_001261282.1 NZ_CP066524:c93642-93412 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT184552 NZ_CP087685:c876-616 [Xylella taiwanensis]
ATGGCTGCAAATGCACTTGTGCGTGCCAGAGTTGATGAGGCTCTTAAAGATGAGGCCGCTTCTGTACTTGCGACGATGGG
CCTTACCGTATCTGATCTTATACGTATCACCTTGACTAAAGTGGCTCGTGAAAAAATGTTGCCTTTTGAGATGCACTCAC
CTAATAGGCTTACGGTTGAGACTTTTGTTAAAAGTGAGTGTGGTGAGGATGTGCATTATGCTGAAAGTGCTGATGCCATG
TTTAAAGATCTTGGTATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References