Detailed information of TA system TA184530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1014..1488
• Replicon: plasmid p3_090329
• Accession: NZ_CP066522
• Organism: Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: H3G99_RS29265
• Protein ID: WP_004197762.1
• Coordinates: 1222..1488 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: H3G99_RS29260
• Protein ID: WP_000879771.1
• Coordinates: 1014..1238 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G99_RS29255	135..347	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
H3G99_RS29260	1014..1238	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
H3G99_RS29265	1222..1488	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H3G99_RS29270	1558..1908	-	351	WP_004197756.1	hypothetical protein	-
H3G99_RS29275	1974..2519	-	546	WP_023317533.1	hypothetical protein	-
H3G99_RS29280	2545..2924	-	380	Protein_6	hypothetical protein	-
H3G99_RS29285	4027..4203	+	177	WP_004197757.1	hypothetical protein	-
H3G99_RS29290	4193..4537	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
H3G99_RS29295	4527..5477	+	951	Protein_9	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T184530 WP_004197762.1 NZ_CP066522:1222-1488 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T184530 NZ_CP087678:2793059-2793475 [Xylella taiwanensis]
TTGAGCGGATATTTGCTCGATACGAACATCATTAGCGACATGATCCGTAATCCGTTTGGACTAGTTGCGTGTCGCATTGA
AAAGGTCGATCCAAAAGAGGTTTGCACTAGCATCGTTGTCGCTGCTGAGTTGCGGTATGGTTGTGCAAAGAAAGGATCGA
CCAAGTTGCTTGCGAAGGTGAAGCTCATGCTTGAAACAATTCCTGTGCTGCCTTTGGATATTCCTACCGACGCTGAATAT
GGTCGCATTCGTGCAGAGTTGGAGTCAGTAGGACAAACTATCGGCTTAAATGATCTATTGATAGGTACTCATGCTTATGT
ACTGGGGATGACATTGGTAACGGATAATGTTAAAGAGTTTAAGCGTATTCGTGGCCTTAAGATCGAAAATTGGCTGGAAT
GTGCATCCTCGGTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT184530 WP_000879771.1 NZ_CP066522:1014-1238 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT184530 NZ_CP087678:2792790-2793062 [Xylella taiwanensis]
ATGTCTATTTCTATGCCATCATCTAGACTCAAGGAAGCTAAGCTGTTCCGCAACAATCGCAGTCAGGCCGTGAGAATTCC
GGTTGAGTTTGAATTACCGGGGGATCGAGTATTGATTCATCGCGAGGGTAGCAAGCTCATCATTGAGCCTGTGAGCATGC
CTACCAACATTATAGAATTGTTAGCGCAGTGGAAAACGGAAGATCCGCTTGGACTTGAAGATCAGTTTCCTGAAATTGAG
GACATGCTTGCTAAGCCGGATGACATATTTTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5