Detailed information of TA system TA184529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2306..2886
• Replicon: plasmid p2_090329
• Accession: NZ_CP066521
• Organism: Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: H3G99_RS29205
• Protein ID: WP_071177730.1
• Coordinates: 2572..2886 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: H3G99_RS29200
• Protein ID: WP_000093040.1
• Coordinates: 2306..2584 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G99_RS29185	879..1124	+	246	WP_032440458.1	hypothetical protein	-
H3G99_RS29190	1398..1769	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
H3G99_RS29195	1766..1972	+	207	Protein_3	TonB family protein	-
H3G99_RS29200	2306..2584	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
H3G99_RS29205	2572..2886	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H3G99_RS29210	3050..3478	+	429	WP_001140599.1	hypothetical protein	-
H3G99_RS29215	3504..3683	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
H3G99_RS29220	3710..4240	-	531	WP_071177729.1	hypothetical protein	-
H3G99_RS29225	4247..4978	-	732	WP_071177728.1	replication-relaxation family protein	-
H3G99_RS29230	4978..6942	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T184529 WP_071177730.1 NZ_CP066521:2572-2886 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T184529 NZ_CP087678:c2627311-2627057 [Xylella taiwanensis]
ATGAAGCGTAAGCACGCTAAGACCCTCAGCACCATAGACACGCGCCCTGTTTCCGCCAACATCCAATGGCGCGATATTGA
AGCACTCTTCTTGGAGCTTGGAGCGACAGTAGAAGAACGTGAAGGGTCTCGTGTGCTGGTGCGGCTGTTCGGTGAACGCC
GTGTATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGCGCTGTTGAATCGATCCGAGCTTGGTTGAAATCGAAC
GGAGTGACACCATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT184529 WP_000093040.1 NZ_CP066521:2306-2584 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT184529 NZ_CP087678:c2627060-2626725 [Xylella taiwanensis]
ATGAAAAACATCATGACCATTGAAGGCTTCAAAGCCATCATTACCTATGATCCGGATATCGACATGTTTCGCGGTGAGTT
TGTCGGTATCAATGGCGGTGCTGATTTCTATGCCAAAGACCTCAAGGGCCTTCGTCGAGAAGGGGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCGAAGAAGATGACGTCGAACCACGCAAATCCTACTCAGGGAAGTTCAATGCAAGGATCTCTCCT
GAACTCCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGAATCAGTTTGAACCAATTTGTAGAACAGGCGATTCAGCA
TGAAATCCACATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1