Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7025..7668 Replicon plasmid pKPC2_090329
Accession NZ_CP066518
Organism Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H3G99_RS26705 Protein ID WP_001044770.1
Coordinates 7252..7668 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H3G99_RS26700 Protein ID WP_001261282.1
Coordinates 7025..7255 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G99_RS26660 2313..2492 + 180 WP_006788217.1 hypothetical protein -
H3G99_RS26665 2988..3782 - 795 WP_004197635.1 site-specific integrase -
H3G99_RS26670 3980..4996 - 1017 WP_017899884.1 hypothetical protein -
H3G99_RS26675 5007..5321 - 315 WP_053389906.1 hypothetical protein -
H3G99_RS26680 5348..5743 - 396 WP_017899885.1 hypothetical protein -
H3G99_RS26685 5912..6217 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
H3G99_RS26690 6219..6437 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
H3G99_RS26695 6607..7068 - 462 WP_160866775.1 hypothetical protein -
H3G99_RS26700 7025..7255 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H3G99_RS26705 7252..7668 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H3G99_RS26710 7742..9304 + 1563 WP_004206609.1 AAA family ATPase -
H3G99_RS26715 9289..10311 + 1023 WP_000361404.1 helicase UvrD -
H3G99_RS26720 10567..11264 + 698 Protein_14 IS1 family transposase -
H3G99_RS26725 11293..11565 + 273 Protein_15 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..149181 149181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T184519 WP_001044770.1 NZ_CP066518:7252-7668 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T184519 NZ_CP087677:c31431-31159 [Xylella taiwanensis]
ATGCGCCGTCCTGCTTATTCTTCGCAGTTCAAGCGTGATGTGCGAGTGTCTCAGAAGCGCGGTAAGGACATAGAGAAACT
TAAGATTCTAATAACTTTGCTGATTGAGGGTAGTCCGTTGCCTGCGATGTATTTGGATCATCCGCTTAAGGGGCAGTGGC
GTAGTTTCCGGGATGCTCATGTTGAGCCGGATTGGTTGTTAATCTATAAGGTTGATGGTGATGTGGTGCGCTTTGAGCGT
ACTGGTCGTCATGCTGATTTGTTCGACGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT184519 WP_001261282.1 NZ_CP066518:7025-7255 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT184519 NZ_CP087677:c31691-31431 [Xylella taiwanensis]
ATGGCTGCAAATGCACTTGTGCGTGCCAGAGTTGATGAGGCTCTTAAAGATGAGGCCGCTTCTGTACTTGCGACGATGGG
CCTTACCGTATCTGATCTTATACGTATCACCTTGACTAAAGTGGCTCGTGAAAAAATGTTGCCTTTTGAGATGCACTCAC
CTAATAGGCTTACGGTTGAGACTTTTGTTAAAAGTGAGTGTGGTGAGGATGTGCATTATGCTGAAAGTGCTGATGCCATG
TTTAAAGATCTTGGTATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References