184518

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5912..6437 Replicon plasmid pKPC2_090329
Accession NZ_CP066518
Organism Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag H3G99_RS26685 Protein ID WP_013023785.1
Coordinates 5912..6217 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H3G99_RS26690 Protein ID WP_001568025.1
Coordinates 6219..6437 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G99_RS26655 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
H3G99_RS26660 2313..2492 + 180 WP_006788217.1 hypothetical protein -
H3G99_RS26665 2988..3782 - 795 WP_004197635.1 site-specific integrase -
H3G99_RS26670 3980..4996 - 1017 WP_017899884.1 hypothetical protein -
H3G99_RS26675 5007..5321 - 315 WP_053389906.1 hypothetical protein -
H3G99_RS26680 5348..5743 - 396 WP_017899885.1 hypothetical protein -
H3G99_RS26685 5912..6217 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
H3G99_RS26690 6219..6437 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H3G99_RS26695 6607..7068 - 462 WP_160866775.1 hypothetical protein -
H3G99_RS26700 7025..7255 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H3G99_RS26705 7252..7668 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
H3G99_RS26710 7742..9304 + 1563 WP_004206609.1 AAA family ATPase -
H3G99_RS26715 9289..10311 + 1023 WP_000361404.1 helicase UvrD -
H3G99_RS26720 10567..11264 + 698 Protein_14 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..149181 149181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T184518 WP_013023785.1 NZ_CP066518:c6217-5912 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T184518 NZ_CP087676:c2665662-2665408 [Xylella taiwanensis]
ATGAAGCGTAAGCACGCTAAGACCCTCAGCACCATAGACACGCGCCCTGTTTCCGCCAACATCCAATGGCGCGATATTGA
AGCACTCTTCTTGGAGCTTGGAGCGACAGTAGAAGAACGTGAAGGGTCTCGTGTGCTGGTGCGGCTGTTCGGTGAACGCC
GTGTATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGCGCTGTTGAATCGATCCGAGCTTGGTTGAAATCGAAC
GGAGTGACACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT184518 WP_001568025.1 NZ_CP066518:c6437-6219 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT184518 NZ_CP087676:c2665411-2665076 [Xylella taiwanensis]
ATGAAAAACATCATGACCATTGAAGGCTTCAAAGCCATCATTACCTATGATCCGGATATCGACATGTTTCGCGGTGAGTT
TGTCGGTATCAATGGCGGTGCTGATTTCTATGCCAAAGACCTCAAGGGCCTTCGTCGAGAAGGGGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCGAAGAAGATGACGTCGAACCACGCAAATCCTACTCAGGGAAGTTCAATGCAAGGATCTCTCCT
GAACTCCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGAATCAGTTTGAACCAATTTGTAGAACAGGCGATTCAGCA
TGAAATCCACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References