Detailed information of TA system TA184499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1279227..1280144
• Replicon: chromosome
• Accession: NZ_CP066501
• Organism: Bacillus velezensis strain ID-A02

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: JEQ22_RS06685
• Protein ID: WP_007407256.1
• Coordinates: 1279398..1280144 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JEQ22_RS06680
• Protein ID: WP_003154807.1
• Coordinates: 1279227..1279397 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ22_RS06630 (JEQ22_06590)	1274433..1274945	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
JEQ22_RS06635 (JEQ22_06595)	1275058..1275609	+	552	Protein_1246	terminase	-
JEQ22_RS06640 (JEQ22_06600)	1275720..1276082	+	363	WP_014721043.1	hypothetical protein	-
JEQ22_RS06645 (JEQ22_06605)	1276095..1276466	+	372	WP_017417608.1	XkdW family protein	-
JEQ22_RS06650 (JEQ22_06610)	1276471..1276668	+	198	WP_007610833.1	XkdX family protein	-
JEQ22_RS06655 (JEQ22_06615)	1276725..1277486	+	762	WP_025649639.1	hypothetical protein	-
JEQ22_RS06660 (JEQ22_06620)	1277538..1277801	+	264	WP_021493821.1	hemolysin XhlA family protein	-
JEQ22_RS06665 (JEQ22_06625)	1277815..1278078	+	264	WP_003154813.1	phage holin	-
JEQ22_RS06670 (JEQ22_06630)	1278092..1278970	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
JEQ22_RS06675	1279005..1279130	-	126	WP_003154809.1	hypothetical protein	-
JEQ22_RS06680 (JEQ22_06635)	1279227..1279397	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JEQ22_RS06685 (JEQ22_06640)	1279398..1280144	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JEQ22_RS06690 (JEQ22_06645)	1280248..1281246	-	999	WP_017417610.1	inorganic phosphate transporter	-
JEQ22_RS06695 (JEQ22_06650)	1281259..1281876	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JEQ22_RS06700 (JEQ22_06655)	1282163..1283479	-	1317	WP_007610842.1	amino acid permease	-
JEQ22_RS06705 (JEQ22_06660)	1283806..1284741	+	936	WP_237586758.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T184499 WP_007407256.1 NZ_CP066501:c1280144-1279398 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T184499 NZ_CP087673:c4772302-4771547 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT184499 WP_003154807.1 NZ_CP066501:c1279397-1279227 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT184499 NZ_CP087673:c4772805-4772437 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14