Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 479126..479763 Replicon chromosome
Accession NZ_CP066501
Organism Bacillus velezensis strain ID-A02

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JEQ22_RS02420 Protein ID WP_003156187.1
Coordinates 479413..479763 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag JEQ22_RS02415 Protein ID WP_003156188.1
Coordinates 479126..479407 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JEQ22_RS02395 (JEQ22_02375) 475491..476090 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
JEQ22_RS02400 (JEQ22_02380) 476183..476548 + 366 WP_007609580.1 holo-ACP synthase -
JEQ22_RS02405 (JEQ22_02385) 476713..477720 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
JEQ22_RS02410 (JEQ22_02390) 477837..479006 + 1170 WP_025649966.1 alanine racemase -
JEQ22_RS02415 (JEQ22_02395) 479126..479407 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JEQ22_RS02420 (JEQ22_02400) 479413..479763 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JEQ22_RS02425 (JEQ22_02405) 479881..480702 + 822 WP_025649964.1 STAS domain-containing protein -
JEQ22_RS02430 (JEQ22_02410) 480707..481072 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
JEQ22_RS02435 (JEQ22_02415) 481075..481476 + 402 WP_003156178.1 anti-sigma regulatory factor -
JEQ22_RS02440 (JEQ22_02420) 481488..482495 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
JEQ22_RS02445 (JEQ22_02425) 482559..482888 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
JEQ22_RS02450 (JEQ22_02430) 482885..483367 + 483 WP_007609591.1 anti-sigma B factor RsbW -
JEQ22_RS02455 (JEQ22_02435) 483333..484121 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
JEQ22_RS02460 (JEQ22_02440) 484121..484723 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T184498 WP_003156187.1 NZ_CP066501:479413-479763 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T184498 NZ_CP087673:3844491-3845069 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTTTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAAGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT184498 WP_003156188.1 NZ_CP066501:479126-479407 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT184498 NZ_CP087673:3843848-3844402 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCATCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGACGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGATGGACGCGCGGTACGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References