Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2145624..2146138 Replicon chromosome
Accession NZ_CP066492
Organism Staphylococcus aureus strain nan_175_F371_ch

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag JF380_RS10745 Protein ID WP_000581792.1
Coordinates 2145624..2145833 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag JF380_RS10750 Protein ID WP_000138932.1
Coordinates 2145845..2146138 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JF380_RS10725 2141132..2142490 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
JF380_RS10730 2142505..2143575 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
JF380_RS10735 2143893..2145095 + 1203 WP_001109939.1 rod shape-determining protein RodA -
JF380_RS10740 2145359..2145496 - 138 WP_000828354.1 hypothetical protein -
JF380_RS10745 2145624..2145833 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
JF380_RS10750 2145845..2146138 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
JF380_RS10755 2146304..2147788 + 1485 WP_000571549.1 cardiolipin synthase -
JF380_RS10760 2147816..2148463 + 648 WP_001187630.1 HD domain-containing protein -
JF380_RS10765 2149175..2150047 - 873 WP_000725800.1 membrane protein insertase YidC -
JF380_RS10770 2150133..2150774 - 642 WP_000483156.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T184491 WP_000581792.1 NZ_CP066492:c2145833-2145624 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T184491 NZ_CP087670:36655-37125 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT184491 WP_000138932.1 NZ_CP066492:c2146138-2145845 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT184491 NZ_CP087670:36215-36658 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References