Detailed information of TA system TA184473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2149262..2149791
• Replicon: chromosome
• Accession: NZ_CP066488
• Organism: Staphylococcus aureus strain ncr_155_F133

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JF379_RS10695
• Protein ID: WP_000621175.1
• Coordinates: 2149262..2149624 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JF379_RS10700
• Protein ID: WP_000948331.1
• Coordinates: 2149621..2149791 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JF379_RS10675	2146241..2147011	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
JF379_RS10680	2146986..2147465	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
JF379_RS10685	2147467..2147793	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JF379_RS10690	2147912..2148913	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JF379_RS10695	2149262..2149624	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JF379_RS10700	2149621..2149791	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JF379_RS10705	2149876..2151024	-	1149	WP_001281154.1	alanine racemase	-
JF379_RS10710	2151090..2151449	-	360	WP_000581197.1	holo-ACP synthase	-
JF379_RS10715	2151453..2151944	-	492	WP_001205912.1	PH domain-containing protein	-
JF379_RS10720	2151931..2153514	-	1584	WP_001294626.1	PH domain-containing protein	-
JF379_RS10725	2153507..2153986	-	480	WP_001287078.1	hypothetical protein	-
JF379_RS10730	2154195..2154755	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T184473 WP_000621175.1 NZ_CP066488:c2149624-2149262 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T184473 NZ_CP087665:c6169-5864 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT184473 WP_000948331.1 NZ_CP066488:c2149791-2149621 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT184473 NZ_CP087665:c6389-6171 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1