Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 25832..26439 Replicon plasmid p93_2_62kb
Accession NZ_CP066476
Organism Enterococcus faecium strain Dallas 93_2

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag JCP72_RS15270 Protein ID WP_002321032.1
Coordinates 26089..26439 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag JCP72_RS15265 Protein ID WP_002287514.1
Coordinates 25832..26095 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP72_RS15225 21182..21655 + 474 WP_002321302.1 single-stranded DNA-binding protein -
JCP72_RS15230 22021..22281 + 261 WP_002321303.1 hypothetical protein -
JCP72_RS15235 22726..22932 + 207 WP_002296239.1 hypothetical protein -
JCP72_RS15240 22932..23183 + 252 WP_002305766.1 hypothetical protein -
JCP72_RS15245 23199..23615 + 417 WP_002305764.1 hypothetical protein -
JCP72_RS15250 23597..23869 + 273 WP_002348912.1 hypothetical protein -
JCP72_RS15255 24036..24437 + 402 WP_002305759.1 IS200/IS605 family transposase -
JCP72_RS15260 24449..25594 + 1146 WP_002305757.1 IS200/IS605 family element transposase accessory protein TnpB -
JCP72_RS15265 25832..26095 + 264 WP_002287514.1 hypothetical protein Antitoxin
JCP72_RS15270 26089..26439 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP72_RS15275 26463..27077 - 615 WP_002318230.1 recombinase family protein -
JCP72_RS15280 27527..28852 + 1326 WP_002348911.1 Y-family DNA polymerase -
JCP72_RS15285 28845..29195 + 351 WP_002303114.1 hypothetical protein -
JCP72_RS15290 29383..29676 + 294 WP_002322251.1 hypothetical protein -
JCP72_RS15295 29902..30720 + 819 WP_002305212.1 ParA family protein -
JCP72_RS15300 30739..31146 + 408 WP_002305774.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..62212 62212


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T184461 WP_002321032.1 NZ_CP066476:26089-26439 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T184461 NZ_CP087663:743107-743592 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT184461 WP_002287514.1 NZ_CP066476:25832-26095 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT184461 NZ_CP087663:742817-743110 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References