Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2197476..2198047 Replicon chromosome
Accession NZ_CP066473
Organism Enterococcus faecium strain Dallas 93_2

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP72_RS10680 Protein ID WP_002286801.1
Coordinates 2197476..2197817 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP72_RS10685 Protein ID WP_002323011.1
Coordinates 2197817..2198047 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP72_RS10660 2192754..2193584 + 831 WP_002286789.1 manganese catalase family protein -
JCP72_RS10665 2193828..2194110 - 283 Protein_2083 transposase -
JCP72_RS10670 2194268..2194462 - 195 WP_002297028.1 hypothetical protein -
JCP72_RS10675 2195256..2196443 + 1188 WP_002296840.1 IS256-like element IS16 family transposase -
JCP72_RS10680 2197476..2197817 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP72_RS10685 2197817..2198047 - 231 WP_002323011.1 hypothetical protein Antitoxin
JCP72_RS10690 2198334..2198672 + 339 WP_002286804.1 hypothetical protein -
JCP72_RS10695 2198877..2199116 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP72_RS10700 2199092..2199439 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP72_RS10705 2200171..2200752 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
JCP72_RS10710 2200935..2201561 - 627 WP_002286816.1 cysteine hydrolase -
JCP72_RS10715 2201583..2202914 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2195256..2196443 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184457 WP_002286801.1 NZ_CP066473:c2197817-2197476 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184457 NZ_CP087659:c6177-5872 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184457 WP_002323011.1 NZ_CP066473:c2198047-2197817 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184457 NZ_CP087659:c6397-6179 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References