Detailed information of TA system TA184450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 48471..48990
• Replicon: plasmid p87_1_57kb
• Accession: NZ_CP066461
• Organism: Enterococcus faecium strain Dallas 87_1

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: JCP85_RS16220
• Protein ID: WP_000588503.1
• Coordinates: 48733..48990 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: JCP85_RS16215
• Protein ID: WP_000388479.1
• Coordinates: 48471..48740 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP85_RS16190	43995..44567	+	573	WP_000170424.1	recombinase family protein	-
JCP85_RS16195	44629..45309	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
JCP85_RS16200	45414..46013	+	600	WP_011117472.1	hypothetical protein	-
JCP85_RS16205	46302..47552	-	1251	WP_002305888.1	IS110 family transposase	-
JCP85_RS16210	48161..48340	+	180	WP_001120991.1	hypothetical protein	-
JCP85_RS16215	48471..48740	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JCP85_RS16220	48733..48990	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
JCP85_RS16225	49449..50453	+	1005	WP_000455809.1	hypothetical protein	-
JCP85_RS16230	50618..51232	-	615	WP_002318230.1	recombinase family protein	-
JCP85_RS16235	51683..53008	+	1326	WP_000969590.1	Y-family DNA polymerase	-
JCP85_RS16240	53001..53351	+	351	WP_000568378.1	hypothetical protein	-
JCP85_RS16245	53663..53953	+	291	WP_000026576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / VanHAX	-	1..57116	57116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T184450 WP_000588503.1 NZ_CP066461:48733-48990 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T184450 NZ_CP087657:c4851750-4851217 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT184450 WP_000388479.1 NZ_CP066461:48471-48740 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT184450 NZ_CP087657:c4852027-4851761 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7