Detailed information of TA system TA184449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 13985..14592
• Replicon: plasmid p87_1_62kb
• Accession: NZ_CP066460
• Organism: Enterococcus faecium strain Dallas 87_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP85_RS15695
• Protein ID: WP_002321032.1
• Coordinates: 13985..14335 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP85_RS15700
• Protein ID: WP_002287514.1
• Coordinates: 14329..14592 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP85_RS15665	9277..9684	-	408	WP_002305774.1	hypothetical protein	-
JCP85_RS15670	9703..10521	-	819	WP_002305212.1	ParA family protein	-
JCP85_RS15675	10747..11040	-	294	WP_002322251.1	hypothetical protein	-
JCP85_RS15680	11228..11578	-	351	WP_002303114.1	hypothetical protein	-
JCP85_RS15685	11571..12896	-	1326	WP_000969590.1	Y-family DNA polymerase	-
JCP85_RS15690	13347..13961	+	615	WP_002318230.1	recombinase family protein	-
JCP85_RS15695	13985..14335	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP85_RS15700	14329..14592	-	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP85_RS15705	14830..15975	-	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP85_RS15710	15987..16388	-	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP85_RS15715	16555..16827	-	273	WP_002348912.1	hypothetical protein	-
JCP85_RS15720	16809..17225	-	417	WP_002305764.1	hypothetical protein	-
JCP85_RS15725	17241..17492	-	252	WP_002305766.1	hypothetical protein	-
JCP85_RS15730	17492..17698	-	207	WP_002296239.1	hypothetical protein	-
JCP85_RS15735	18143..18403	-	261	WP_002321303.1	hypothetical protein	-
JCP85_RS15740	18769..19242	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..62213	62213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184449 WP_002321032.1 NZ_CP066460:c14335-13985 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184449 NZ_CP087657:4238151-4238369 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184449 WP_002287514.1 NZ_CP066460:c14592-14329 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184449 NZ_CP087657:4237750-4238124 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0