Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 541262..541833 Replicon chromosome
Accession NZ_CP066457
Organism Enterococcus faecium strain Dallas 87_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP85_RS02550 Protein ID WP_002286801.1
Coordinates 541492..541833 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP85_RS02545 Protein ID WP_002323011.1
Coordinates 541262..541492 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP85_RS02515 536395..537726 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
JCP85_RS02520 537748..538374 + 627 WP_002286816.1 cysteine hydrolase -
JCP85_RS02525 538557..539138 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
JCP85_RS02530 539870..540217 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP85_RS02535 540193..540432 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP85_RS02540 540637..540975 - 339 WP_002286804.1 hypothetical protein -
JCP85_RS02545 541262..541492 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP85_RS02550 541492..541833 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP85_RS02555 542866..544053 - 1188 WP_002296840.1 IS256-like element IS16 family transposase -
JCP85_RS02560 544847..545041 + 195 WP_002297028.1 hypothetical protein -
JCP85_RS02565 545199..545481 + 283 Protein_509 transposase -
JCP85_RS02570 545725..546555 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 542866..544053 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184447 WP_002286801.1 NZ_CP066457:541492-541833 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184447 NZ_CP087657:2056010-2056112 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184447 WP_002323011.1 NZ_CP066457:541262-541492 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184447 NZ_CP087657:c2056119-2055974 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References