Detailed information of TA system TA184428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 517243..517814
• Replicon: chromosome
• Accession: NZ_CP066434
• Organism: Enterococcus faecium strain Dallas 66

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3Y2B8
• Locus tag: JCP89_RS02475
• Protein ID: WP_002286801.1
• Coordinates: 517473..517814 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: JCP89_RS02470
• Protein ID: WP_002323011.1
• Coordinates: 517243..517473 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP89_RS02440	512376..513707	+	1332	WP_002286818.1	FAD-containing oxidoreductase	-
JCP89_RS02445	513729..514355	+	627	WP_002286816.1	cysteine hydrolase	-
JCP89_RS02450	514538..515119	+	582	WP_010729547.1	TetR/AcrR family transcriptional regulator	-
JCP89_RS02455	515851..516198	+	348	WP_002286808.1	SOS response-associated peptidase family protein	-
JCP89_RS02460	516174..516413	+	240	WP_002321938.1	SOS response-associated peptidase family protein	-
JCP89_RS02465	516618..516956	-	339	WP_002286804.1	hypothetical protein	-
JCP89_RS02470	517243..517473	+	231	WP_002323011.1	hypothetical protein	Antitoxin
JCP89_RS02475	517473..517814	+	342	WP_002286801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP89_RS02480	518664..518849	+	186	WP_002304207.1	hypothetical protein	-
JCP89_RS02485	519354..519548	+	195	WP_002297028.1	hypothetical protein	-
JCP89_RS02490	519706..519988	+	283	Protein_495	transposase	-
JCP89_RS02495	520232..521062	-	831	WP_002286789.1	manganese catalase family protein	-
JCP89_RS02500	521270..522604	+	1335	WP_002286788.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13302.68 Da
• Isoelectric Point: 9.9044

>T184428 WP_002286801.1 NZ_CP066434:517473-517814 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T184428 NZ_CP087651:743189-743674 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT184428 WP_002323011.1 NZ_CP066434:517243-517473 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT184428 NZ_CP087651:742899-743192 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A829FD66

Antitoxin

• AlphaFold DB: A0A828ZZN9