Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2423608..2424179 Replicon chromosome
Accession NZ_CP066421
Organism Enterococcus faecium strain Dallas 55

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP69_RS11710 Protein ID WP_002286801.1
Coordinates 2423608..2423949 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP69_RS11715 Protein ID WP_002323011.1
Coordinates 2423949..2424179 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP69_RS11690 2418886..2419716 + 831 WP_002286789.1 manganese catalase family protein -
JCP69_RS11695 2419960..2420242 - 283 Protein_2291 transposase -
JCP69_RS11700 2420400..2420594 - 195 WP_002297028.1 hypothetical protein -
JCP69_RS11705 2421388..2422575 + 1188 WP_002296840.1 IS256-like element IS16 family transposase -
JCP69_RS11710 2423608..2423949 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP69_RS11715 2423949..2424179 - 231 WP_002323011.1 hypothetical protein Antitoxin
JCP69_RS11720 2424466..2424804 + 339 WP_002286804.1 hypothetical protein -
JCP69_RS11725 2425009..2425248 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP69_RS11730 2425224..2425571 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP69_RS11735 2426303..2426884 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
JCP69_RS11740 2427067..2427693 - 627 WP_002286816.1 cysteine hydrolase -
JCP69_RS11745 2427715..2429046 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2421388..2422575 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184418 WP_002286801.1 NZ_CP066421:c2423949-2423608 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184418 NZ_CP087645:c4815998-4815465 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184418 WP_002323011.1 NZ_CP066421:c2424179-2423949 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184418 NZ_CP087645:c4816275-4816009 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References