Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 515618..516189 Replicon chromosome
Accession NZ_CP066415
Organism Enterococcus faecium strain Dallas 53_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP73_RS02470 Protein ID WP_002286801.1
Coordinates 515848..516189 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP73_RS02465 Protein ID WP_002323011.1
Coordinates 515618..515848 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP73_RS02435 510751..512082 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
JCP73_RS02440 512104..512730 + 627 WP_002286816.1 cysteine hydrolase -
JCP73_RS02445 512913..513494 + 582 WP_010729547.1 TetR/AcrR family transcriptional regulator -
JCP73_RS02450 514226..514573 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP73_RS02455 514549..514788 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP73_RS02460 514993..515331 - 339 WP_002286804.1 hypothetical protein -
JCP73_RS02465 515618..515848 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP73_RS02470 515848..516189 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP73_RS02475 517039..517224 + 186 WP_002304207.1 hypothetical protein -
JCP73_RS02480 517729..517923 + 195 WP_002297028.1 hypothetical protein -
JCP73_RS02485 518081..518363 + 283 Protein_494 transposase -
JCP73_RS02490 518607..519437 - 831 WP_002286789.1 manganese catalase family protein -
JCP73_RS02495 519645..520979 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184412 WP_002286801.1 NZ_CP066415:515848-516189 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184412 NZ_CP087645:357447-357794 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184412 WP_002323011.1 NZ_CP066415:515618-515848 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184412 NZ_CP087645:357794-358093 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References