Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 515619..516190 Replicon chromosome
Accession NZ_CP066409
Organism Enterococcus faecium strain Dallas 53_2

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP94_RS02460 Protein ID WP_002286801.1
Coordinates 515849..516190 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP94_RS02455 Protein ID WP_002323011.1
Coordinates 515619..515849 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP94_RS02425 510752..512083 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
JCP94_RS02430 512105..512731 + 627 WP_002286816.1 cysteine hydrolase -
JCP94_RS02435 512914..513495 + 582 WP_010729547.1 TetR/AcrR family transcriptional regulator -
JCP94_RS02440 514227..514574 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP94_RS02445 514550..514789 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP94_RS02450 514994..515332 - 339 WP_002286804.1 hypothetical protein -
JCP94_RS02455 515619..515849 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP94_RS02460 515849..516190 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP94_RS02465 517040..517225 + 186 WP_002304207.1 hypothetical protein -
JCP94_RS02470 517730..517924 + 195 WP_002297028.1 hypothetical protein -
JCP94_RS02475 518082..518364 + 283 Protein_492 transposase -
JCP94_RS02480 518608..519438 - 831 WP_002286789.1 manganese catalase family protein -
JCP94_RS02485 519646..520980 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184406 WP_002286801.1 NZ_CP066409:515849-516190 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184406 NZ_CP087641:19053-19469 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184406 WP_002323011.1 NZ_CP066409:515619-515849 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184406 NZ_CP087641:18826-19056 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References