184402

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 568..1676 Replicon plasmid p51_4_29kb
Accession NZ_CP066405
Organism Enterococcus faecium strain Dallas 51_4

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag JCQ06_RS16055 Protein ID WP_002303392.1
Coordinates 807..1676 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag JCQ06_RS16050 Protein ID WP_002303393.1
Coordinates 568..792 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCQ06_RS16045 120..425 + 306 WP_099097609.1 site-specific recombinase -
JCQ06_RS16050 568..792 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
JCQ06_RS16055 807..1676 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
JCQ06_RS16060 1657..2391 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
JCQ06_RS16065 2424..3332 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
JCQ06_RS16070 3329..3577 + 249 WP_002347175.1 hypothetical protein -
JCQ06_RS16075 3580..4488 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
JCQ06_RS16080 4589..5884 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
JCQ06_RS16085 6129..6380 + 252 Protein_8 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / aph(3')-III / erm(B) / lnu(B) - 1..29903 29903
- inside IScluster/Tn ant(6)-Ia / aph(3')-III / erm(B) / lnu(B) - 2424..29900 27476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T184402 WP_002303392.1 NZ_CP066405:807-1676 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T184402 NZ_CP087639:c4913801-4913517 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT184402 WP_002303393.1 NZ_CP066405:568-792 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT184402 NZ_CP087639:c4914033-4913791 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References