Detailed information of TA system TA184397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 44244..44851
• Replicon: plasmid p17_1_56kb
• Accession: NZ_CP066391
• Organism: Enterococcus faecium strain Dallas 17_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP93_RS14920
• Protein ID: WP_002321032.1
• Coordinates: 44244..44594 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP93_RS14925
• Protein ID: WP_002287514.1
• Coordinates: 44588..44851 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP93_RS14890	39506..39799	-	294	WP_002322251.1	hypothetical protein	-
JCP93_RS14895	39811..39990	-	180	WP_002322386.1	hypothetical protein	-
JCP93_RS14900	39987..40337	-	351	WP_002303114.1	hypothetical protein	-
JCP93_RS14905	40330..41655	-	1326	WP_002348911.1	Y-family DNA polymerase	-
JCP93_RS14910	42105..42719	+	615	WP_002318230.1	recombinase family protein	-
JCP93_RS14915	42845..44146	+	1302	WP_002302440.1	ISL3 family transposase	-
JCP93_RS14920	44244..44594	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP93_RS14925	44588..44851	-	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP93_RS14930	45089..46234	-	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP93_RS14935	46246..46647	-	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP93_RS14940	46814..47086	-	273	WP_002348912.1	hypothetical protein	-
JCP93_RS14945	47068..47484	-	417	WP_002305764.1	hypothetical protein	-
JCP93_RS14950	47500..47751	-	252	WP_002305766.1	hypothetical protein	-
JCP93_RS14955	47751..47957	-	207	WP_002296239.1	hypothetical protein	-
JCP93_RS14960	48402..48662	-	261	WP_002321303.1	hypothetical protein	-
JCP93_RS14965	49028..49501	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56323	56323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184397 WP_002321032.1 NZ_CP066391:c44594-44244 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184397 NZ_CP087639:879134-879541 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184397 WP_002287514.1 NZ_CP066391:c44851-44588 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184397 NZ_CP087639:878887-879153 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0