Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 499901..500472 Replicon chromosome
Accession NZ_CP066389
Organism Enterococcus faecium strain Dallas 17_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP93_RS02380 Protein ID WP_002286801.1
Coordinates 500131..500472 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP93_RS02375 Protein ID WP_002323011.1
Coordinates 499901..500131 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP93_RS02345 495034..496365 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
JCP93_RS02350 496387..497013 + 627 WP_002286816.1 cysteine hydrolase -
JCP93_RS02355 497196..497777 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
JCP93_RS02360 498509..498856 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP93_RS02365 498832..499071 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP93_RS02370 499276..499614 - 339 WP_002286804.1 hypothetical protein -
JCP93_RS02375 499901..500131 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP93_RS02380 500131..500472 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP93_RS02385 501322..501507 + 186 WP_002304207.1 hypothetical protein -
JCP93_RS02390 502012..502206 + 195 WP_002297028.1 hypothetical protein -
JCP93_RS02395 502364..502646 + 283 Protein_475 transposase -
JCP93_RS02400 502890..503720 - 831 WP_002286789.1 manganese catalase family protein -
JCP93_RS02405 503928..505262 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184395 WP_002286801.1 NZ_CP066389:500131-500472 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184395 NZ_CP087639:354072-354419 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184395 WP_002323011.1 NZ_CP066389:499901-500131 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184395 NZ_CP087639:354419-354718 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References