Detailed information of TA system TA184391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 49441..50048
• Replicon: plasmid p16_1_257kb
• Accession: NZ_CP066382
• Organism: Enterococcus faecium strain Dallas 16_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP87_RS13710
• Protein ID: WP_002321032.1
• Coordinates: 49698..50048 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP87_RS13705
• Protein ID: WP_002287514.1
• Coordinates: 49441..49704 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP87_RS13665	44477..45376	+	900	WP_198874308.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP87_RS13670	45379..45864	+	486	WP_198874309.1	single-stranded DNA-binding protein	-
JCP87_RS13675	46230..46490	+	261	WP_002296240.1	hypothetical protein	-
JCP87_RS13680	46931..47137	+	207	WP_002296239.1	hypothetical protein	-
JCP87_RS13685	47137..47388	+	252	WP_002296238.1	hypothetical protein	-
JCP87_RS13690	47403..47840	+	438	WP_002296237.1	hypothetical protein	-
JCP87_RS13695	47833..48540	+	708	WP_002353391.1	hypothetical protein	-
JCP87_RS13700	48921..49100	+	180	WP_002305761.1	hypothetical protein	-
JCP87_RS13705	49441..49704	+	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP87_RS13710	49698..50048	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP87_RS13715	50072..50686	-	615	WP_010729795.1	recombinase family protein	-
JCP87_RS13720	51000..51422	+	423	WP_002288787.1	hypothetical protein	-
JCP87_RS13725	51432..51635	+	204	WP_002299573.1	hypothetical protein	-
JCP87_RS13730	51846..52430	+	585	WP_002299575.1	recombinase family protein	-
JCP87_RS13735	52619..53299	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
JCP87_RS13740	53353..54063	-	711	WP_010729797.1	GntR family transcriptional regulator	-
JCP87_RS13745	54047..54781	-	735	WP_010729798.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..257742	257742

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184391 WP_002321032.1 NZ_CP066382:49698-50048 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184391 NZ_CP087636:c18683-18438 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGGAAAAATGATAAATTGCTGGAAAAATTTATAAACAGCAAGAAAACATTTGAATGGACAGAACTGGTTGTACTGCT
TTCATTTCTTGGTTATGAAAAAAAGGAAATGCAGGGATCACGAGTCAGATTTGTACACGAGAAATACAAAACGATAATGC
TACATCGCCCACACCCTGAAAATCATATCAAAGGCGGGGCGCTGAAAGATGTCAAGAGGATGTTAAAAGAGGAAGGTTTA
TTATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184391 WP_002287514.1 NZ_CP066382:49441-49704 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184391 NZ_CP087636:c18441-18091 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACATTTAAAATATAAAGGCTATTTGGGTACAGTAGAGCCAGATTTTGATAATAATATCCTGTATGGAAAACTGGC
GTTTATTGGCGATCTGGTTACTTATGAGGCTGAAACATTAGCAGAACTGGAACAGGAATTTAAAACATCGGTAGATTTGT
ATCTACAGGCTTGTGCGGAGGACGGGAAAAAACCAGACACCCCATTTAAAGGCGTGTTTAATGTGAGGGTAGATCCCGAT
CTGCATCGTCGTATTGCAGAAATGGCGCTTGCAGAAGACTTATCGCTAAATGCGTTTGTTAATAAGGCGCTGGAAAAAGA
AGTTAGTCATAATAGCTATGGGGCTTCATGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0