Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 521297..521868 Replicon chromosome
Accession NZ_CP066381
Organism Enterococcus faecium strain Dallas 16_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP87_RS02505 Protein ID WP_002286801.1
Coordinates 521527..521868 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP87_RS02500 Protein ID WP_002323011.1
Coordinates 521297..521527 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP87_RS02470 516430..517761 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
JCP87_RS02475 517783..518409 + 627 WP_002286816.1 cysteine hydrolase -
JCP87_RS02480 518592..519173 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
JCP87_RS02485 519905..520252 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP87_RS02490 520228..520467 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP87_RS02495 520672..521010 - 339 WP_002286804.1 hypothetical protein -
JCP87_RS02500 521297..521527 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP87_RS02505 521527..521868 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP87_RS02510 522718..522903 + 186 WP_002304207.1 hypothetical protein -
JCP87_RS02515 523408..523602 + 195 WP_002297028.1 hypothetical protein -
JCP87_RS02520 523760..524042 + 283 Protein_500 transposase -
JCP87_RS02525 524286..525116 - 831 WP_002286789.1 manganese catalase family protein -
JCP87_RS02530 525324..526658 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184390 WP_002286801.1 NZ_CP066381:521527-521868 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184390 NZ_CP087635:75115-75264 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184390 WP_002323011.1 NZ_CP066381:521297-521527 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184390 NZ_CP087635:c75070-75011 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References