Detailed information of TA system TA184386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1345872..1346789
• Replicon: chromosome
• Accession: NZ_CP066379
• Organism: Bacillus velezensis strain ID-A04

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: JEQ24_RS07205
• Protein ID: WP_007407256.1
• Coordinates: 1346043..1346789 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JEQ24_RS07200
• Protein ID: WP_003154807.1
• Coordinates: 1345872..1346042 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ24_RS07160 (1341105)	1341105..1342727	+	1623	WP_022553476.1	pyocin knob domain-containing protein	-
JEQ24_RS07165 (1342740)	1342740..1343111	+	372	WP_022553475.1	XkdW family protein	-
JEQ24_RS07170 (1343116)	1343116..1343313	+	198	WP_007610833.1	XkdX family protein	-
JEQ24_RS07175 (1343370)	1343370..1344131	+	762	WP_022553474.1	hypothetical protein	-
JEQ24_RS07180 (1344183)	1344183..1344446	+	264	WP_022553473.1	hemolysin XhlA family protein	-
JEQ24_RS07185 (1344460)	1344460..1344723	+	264	WP_003154813.1	phage holin	-
JEQ24_RS07190 (1344737)	1344737..1345615	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
JEQ24_RS07195 (1345650)	1345650..1345775	-	126	WP_003154809.1	hypothetical protein	-
JEQ24_RS07200 (1345872)	1345872..1346042	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JEQ24_RS07205 (1346043)	1346043..1346789	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JEQ24_RS07210 (1346894)	1346894..1347892	-	999	WP_003154805.1	inorganic phosphate transporter	-
JEQ24_RS07215 (1347905)	1347905..1348522	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JEQ24_RS07220 (1348808)	1348808..1350124	-	1317	WP_007610842.1	amino acid permease	-
JEQ24_RS07225 (1350446)	1350446..1351396	+	951	WP_022553472.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1300653..1404890	104237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T184386 WP_007407256.1 NZ_CP066379:c1346789-1346043 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T184386 NZ_CP087634:c4939307-4939023 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT184386 WP_003154807.1 NZ_CP066379:c1346042-1345872 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT184386 NZ_CP087634:c4939539-4939297 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14