Detailed information of TA system TA184384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1387476..1388393
• Replicon: chromosome
• Accession: NZ_CP066378
• Organism: Bacillus velezensis strain ID-A03

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: JEQ23_RS07495
• Protein ID: WP_007407256.1
• Coordinates: 1387647..1388393 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JEQ23_RS07490
• Protein ID: WP_003154807.1
• Coordinates: 1387476..1387646 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ23_RS07450 (1382709)	1382709..1384331	+	1623	WP_022553476.1	pyocin knob domain-containing protein	-
JEQ23_RS07455 (1384344)	1384344..1384715	+	372	WP_022553475.1	XkdW family protein	-
JEQ23_RS07460 (1384720)	1384720..1384917	+	198	WP_007610833.1	XkdX family protein	-
JEQ23_RS07465 (1384974)	1384974..1385735	+	762	WP_022553474.1	hypothetical protein	-
JEQ23_RS07470 (1385787)	1385787..1386050	+	264	WP_022553473.1	hemolysin XhlA family protein	-
JEQ23_RS07475 (1386064)	1386064..1386327	+	264	WP_003154813.1	phage holin	-
JEQ23_RS07480 (1386341)	1386341..1387219	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
JEQ23_RS07485 (1387254)	1387254..1387379	-	126	WP_003154809.1	hypothetical protein	-
JEQ23_RS07490 (1387476)	1387476..1387646	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JEQ23_RS07495 (1387647)	1387647..1388393	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JEQ23_RS07500 (1388498)	1388498..1389496	-	999	WP_003154805.1	inorganic phosphate transporter	-
JEQ23_RS07505 (1389509)	1389509..1390126	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JEQ23_RS07510 (1390412)	1390412..1391728	-	1317	WP_007610842.1	amino acid permease	-
JEQ23_RS07515 (1392050)	1392050..1393000	+	951	WP_022553472.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T184384 WP_007407256.1 NZ_CP066378:c1388393-1387647 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T184384 NZ_CP087634:4221465-4221683 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT184384 WP_003154807.1 NZ_CP066378:c1387646-1387476 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT184384 NZ_CP087634:4221064-4221438 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14