Detailed information of TA system TA184382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1254282..1255199
• Replicon: chromosome
• Accession: NZ_CP066377
• Organism: Bacillus velezensis strain ID-A01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: JEQ16_RS06660
• Protein ID: WP_057079950.1
• Coordinates: 1254453..1255199 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JEQ16_RS06655
• Protein ID: WP_003154807.1
• Coordinates: 1254282..1254452 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEQ16_RS06615 (JEQ16_06580)	1249515..1251137	+	1623	WP_278071775.1	pyocin knob domain-containing protein	-
JEQ16_RS06620 (JEQ16_06585)	1251150..1251521	+	372	WP_102421824.1	XkdW family protein	-
JEQ16_RS06625 (JEQ16_06590)	1251526..1251723	+	198	WP_070081682.1	XkdX family protein	-
JEQ16_RS06630 (JEQ16_06595)	1251780..1252541	+	762	WP_278071776.1	phage portal protein	-
JEQ16_RS06635 (JEQ16_06600)	1252593..1252856	+	264	WP_003154815.1	hemolysin XhlA family protein	-
JEQ16_RS06640 (JEQ16_06605)	1252870..1253133	+	264	WP_003154813.1	phage holin	-
JEQ16_RS06645 (JEQ16_06610)	1253147..1254025	+	879	WP_058905962.1	N-acetylmuramoyl-L-alanine amidase	-
JEQ16_RS06650	1254060..1254185	-	126	WP_003154809.1	hypothetical protein	-
JEQ16_RS06655 (JEQ16_06615)	1254282..1254452	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JEQ16_RS06660 (JEQ16_06620)	1254453..1255199	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JEQ16_RS06665 (JEQ16_06625)	1255304..1256302	-	999	WP_003154805.1	inorganic phosphate transporter	-
JEQ16_RS06670 (JEQ16_06630)	1256315..1256932	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JEQ16_RS06675 (JEQ16_06635)	1257219..1258535	-	1317	WP_172614618.1	amino acid permease	-
JEQ16_RS06680 (JEQ16_06640)	1258858..1259808	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T184382 WP_057079950.1 NZ_CP066377:c1255199-1254453 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T184382 NZ_CP087634:2026776-2026878 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT184382 WP_003154807.1 NZ_CP066377:c1254452-1254282 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT184382 NZ_CP087634:c2026885-2026740 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: I2HQ14