Detailed information of TA system TA184331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 824816..825501
• Replicon: plasmid pSymA
• Accession: NZ_CP066361
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX10_RS29535
• Protein ID: WP_017263896.1
• Coordinates: 825082..825501 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: JFX10_RS29530
• Protein ID: WP_013845417.1
• Coordinates: 824816..825085 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS29510	820333..821202	-	870	WP_013845421.1	aldo/keto reductase family oxidoreductase	-
JFX10_RS29515	821319..822242	+	924	WP_013845420.1	LysR family transcriptional regulator	-
JFX10_RS29520	823222..824097	+	876	WP_013845418.1	LysR family transcriptional regulator	-
JFX10_RS29525	824208..824678	-	471	Protein_790	LysR family transcriptional regulator	-
JFX10_RS29530	824816..825085	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JFX10_RS29535	825082..825501	+	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX10_RS29540	825655..825894	-	240	Protein_793	hypothetical protein	-
JFX10_RS29545	826165..826476	+	312	WP_026031097.1	type II toxin-antitoxin system HigB family toxin	-
JFX10_RS29550	826494..826874	+	381	WP_003526626.1	transcriptional regulator	-
JFX10_RS29555	827206..828633	+	1428	WP_198868827.1	ISNCY family transposase	-
JFX10_RS29560	828989..829426	+	438	Protein_797	helix-turn-helix domain containing protein	-
JFX10_RS29565	829353..829847	-	495	Protein_798	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB / htpB / katA	1..1580177	1580177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T184331 WP_017263896.1 NZ_CP066361:825082-825501 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T184331 NZ_CP087618:842075-842485 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT184331 WP_013845417.1 NZ_CP066361:824816-825085 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT184331 NZ_CP087618:841828-842094 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5