Detailed information of TA system TA184330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 481697..482196
• Replicon: plasmid pSymA
• Accession: NZ_CP066361
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: JFX10_RS27930
• Protein ID: WP_010967939.1
• Coordinates: 482023..482196 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A843ZXX9
• Locus tag: JFX10_RS27925
• Protein ID: WP_014990215.1
• Coordinates: 481697..482026 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS27890	477072..477248	+	177	WP_013845643.1	hypothetical protein	-
JFX10_RS27895	477374..478267	-	894	WP_010967943.1	LysR family transcriptional regulator	-
JFX10_RS27900	478359..478802	+	444	WP_010967942.1	PACE efflux transporter	-
JFX10_RS27905	478919..479257	+	339	WP_013845641.1	SDR family oxidoreductase	-
JFX10_RS27910	479241..479582	-	342	Protein_467	hypothetical protein	-
JFX10_RS27915	479966..481336	-	1371	WP_198869101.1	alpha/beta hydrolase	-
JFX10_RS27920	481577..481762	-	186	WP_013845639.1	hypothetical protein	-
JFX10_RS27925	481697..482026	+	330	WP_014990215.1	hypothetical protein	Antitoxin
JFX10_RS27930	482023..482196	+	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX10_RS27935	482294..482599	-	306	WP_013845638.1	hypothetical protein	-
JFX10_RS27940	482918..483277	-	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
JFX10_RS27945	483872..484123	+	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
JFX10_RS27950	484151..484636	+	486	WP_010967936.1	YidB family protein	-
JFX10_RS27955	485015..486331	-	1317	WP_013845634.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB / htpB / katA	1..1580177	1580177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T184330 WP_010967939.1 NZ_CP066361:482023-482196 [Sinorhizobium meliloti]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T184330 NZ_CP087618:694374-694715 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12067.72 Da
• Isoelectric Point: 11.1401

>AT184330 WP_014990215.1 NZ_CP066361:481697-482026 [Sinorhizobium meliloti]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQTAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKVLRALEDT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT184330 NZ_CP087618:694036-694353 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Structures

Toxin

• AlphaFold DB: Q92YA6

Antitoxin

• AlphaFold DB: A0A843ZXX9