Detailed information of TA system TA184328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 72113..72648
• Replicon: plasmid pSymA
• Accession: NZ_CP066361
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JFX10_RS25885
• Protein ID: WP_014989489.1
• Coordinates: 72113..72388 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N8JT21
• Locus tag: JFX10_RS25890
• Protein ID: WP_013845222.1
• Coordinates: 72388..72648 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS25860	67501..68448	+	948	WP_198869037.1	LysR family transcriptional regulator	-
JFX10_RS25865	68604..68909	-	306	Protein_57	hypothetical protein	-
JFX10_RS25870	69622..70059	+	438	WP_013845218.1	VOC family protein	-
JFX10_RS25875	70562..70861	+	300	WP_198869038.1	hypothetical protein	-
JFX10_RS25880	70910..72064	-	1155	WP_198869039.1	site-specific integrase	-
JFX10_RS25885	72113..72388	-	276	WP_014989489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX10_RS25890	72388..72648	-	261	WP_013845222.1	ribbon-helix-helix domain-containing protein	Antitoxin
JFX10_RS25895	72803..73012	-	210	Protein_63	transposase	-
JFX10_RS25900	73020..73439	-	420	WP_013845223.1	type II toxin-antitoxin system VapC family toxin	-
JFX10_RS25905	73436..73678	-	243	WP_013845224.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JFX10_RS25910	73900..75036	+	1137	WP_198869040.1	DUF1612 and helix-turn-helix domain-containing protein	-
JFX10_RS25915	75101..75913	-	813	WP_198869041.1	hypothetical protein	-
JFX10_RS25920	76033..77457	-	1425	WP_198869042.1	UvrD-helicase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIc	htpB / htpB / katA	1..1580177	1580177
	-	flank	IS/Tn	-	-	72803..73030	227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10895.46 Da
• Isoelectric Point: 9.9707

>T184328 WP_014989489.1 NZ_CP066361:c72388-72113 [Sinorhizobium meliloti]
MKLEWTSKAVADLGRLYDFLAPVNRQAAARTVQSWTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T184328 NZ_CP087616:8818-9132 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9652.01 Da
• Isoelectric Point: 4.8182

>AT184328 WP_013845222.1 NZ_CP066361:c72648-72388 [Sinorhizobium meliloti]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIDHQAVQAWADSLDSDK
PLSLPL

Nucleotide

• Length: 261 bp

>AT184328 NZ_CP087616:8552-8830 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N8JT21