Detailed information of TA system TA184326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3540972..3541633
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: JFX10_RS24605
• Protein ID: WP_010970565.1
• Coordinates: 3540972..3541376 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: JFX10_RS24610
• Protein ID: WP_010970566.1
• Coordinates: 3541373..3541633 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS24590	3536515..3537627	+	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-
JFX10_RS24595	3537756..3538463	+	708	WP_010970563.1	phosphatase PAP2 family protein	-
JFX10_RS24600	3538564..3540453	-	1890	WP_014527006.1	MHS family MFS transporter	-
JFX10_RS24605	3540972..3541376	-	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX10_RS24610	3541373..3541633	-	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JFX10_RS33255	3541762..3541932	+	171	WP_010970567.1	hypothetical protein	-
JFX10_RS24615	3542046..3543080	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
JFX10_RS24620	3543253..3544074	+	822	WP_010970568.1	lytic transglycosylase family protein	-
JFX10_RS24625	3544397..3545560	-	1164	WP_003537933.1	IS4-like element ISRm16 family transposase	-
JFX10_RS24630	3545729..3546412	-	684	WP_010970569.1	carbonic anhydrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3544397..3545560	1163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T184326 WP_010970565.1 NZ_CP066359:c3541376-3540972 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T184326 NZ_CP087614:c19454-19167 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACATATACCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGAACAAGCTGGATAAGACTATTCAGCAGCAGTTTGC
AAAGAAACTTAAAAAGTGCTGTGAAAACCCACACATTCCTTCAGCAAAACTACGTGGTATAAAGGATTGCTATAAAATAA
AACTGCGAGCATCTGGTTTTCGCCTCGTCTATCAGGTCATAGATAATCAACTTATAATTGCTATTGTGGCTGTAGGCAAA
CGGGAGCGAAGTGACGTTTATACACTGGCGAGTGAACGTATGAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT184326 WP_010970566.1 NZ_CP066359:c3541633-3541373 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT184326 NZ_CP087614:c19702-19451 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACATATCATTCTGAGTGACACGACTGCCAGCGTCAGTGAATTGAAAAAAAACCCGATGGCCACAGTCAGCGCCGG
TGACGGGTTCCCAGTAGCGATTCTTAACCGGAACCAGCCAGCTTTCTATTGCGTGCCTGCGGAACTCTACGAAAAGATGC
TTGATGCGCTGGATGATCAGGAACTCATTAAACTGGTTGCAGAACGCAGTAATCAACCGCTGTTTGATGTGGACCTGGAT
AAGTACCTATGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4