Detailed information of TA system TA184325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2897968..2898599
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX10_RS21725
• Protein ID: WP_013844828.1
• Coordinates: 2897968..2898366 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: JFX10_RS21730
• Protein ID: WP_010970129.1
• Coordinates: 2898366..2898599 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS21690	2893047..2893349	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JFX10_RS21695	2893346..2893621	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
JFX10_RS21700	2893910..2894059	+	150	WP_010970124.1	hypothetical protein	-
JFX10_RS21705	2894056..2894991	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
JFX10_RS21710	2895055..2896008	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
JFX10_RS21715	2896253..2897695	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
JFX10_RS21720	2897695..2897934	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
JFX10_RS21725	2897968..2898366	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
JFX10_RS21730	2898366..2898599	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JFX10_RS21735	2898708..2899853	-	1146	WP_010970130.1	GTP-binding protein	-
JFX10_RS21740	2899867..2900949	-	1083	WP_017268305.1	D-alanyl-D-alanine carboxypeptidase	-
JFX10_RS21745	2901201..2902364	+	1164	WP_010970132.1	amidohydrolase	-
JFX10_RS21750	2902387..2902851	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T184325 WP_013844828.1 NZ_CP066359:c2898366-2897968 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T184325 NZ_CP087614:2149-2481 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGCGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT184325 WP_010970129.1 NZ_CP066359:c2898599-2898366 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT184325 NZ_CP087614:1904-2149 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCGGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTAA

Structures

Antitoxin

• AlphaFold DB: Q92MD9