Detailed information of TA system TA184324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2850418..2851184
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: JFX10_RS21455
• Protein ID: WP_003533488.1
• Coordinates: 2850702..2851184 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: JFX10_RS21450
• Protein ID: WP_003533490.1
• Coordinates: 2850418..2850702 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS21435	2845888..2847276	+	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-
JFX10_RS21440	2847280..2848551	+	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
JFX10_RS21445	2848567..2850213	-	1647	WP_010970088.1	AMP-binding protein	-
JFX10_RS21450	2850418..2850702	+	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
JFX10_RS21455	2850702..2851184	+	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
JFX10_RS21465	2851649..2852158	+	510	WP_013844819.1	disulfide bond formation protein B	-
JFX10_RS21470	2852190..2852747	-	558	WP_003533486.1	HNH endonuclease	-
JFX10_RS21475	2852853..2853500	-	648	WP_010970091.1	DNA-3-methyladenine glycosylase	-
JFX10_RS21480	2853653..2854549	+	897	WP_010970092.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
JFX10_RS21485	2854568..2855443	-	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T184324 WP_003533488.1 NZ_CP066359:2850702-2851184 [Sinorhizobium meliloti]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T184324 NZ_CP087613:7236-7652 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT184324 WP_003533490.1 NZ_CP066359:2850418-2850702 [Sinorhizobium meliloti]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT184324 NZ_CP087613:7009-7239 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4