Detailed information of TA system TA184323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2619187..2619860
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX10_RS20330
• Protein ID: WP_010969926.1
• Coordinates: 2619456..2619860 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: JFX10_RS20325
• Protein ID: WP_003536345.1
• Coordinates: 2619187..2619459 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS20305	2615178..2616818	-	1641	WP_003536336.1	ABC transporter substrate-binding protein	-
JFX10_RS20310	2616866..2617921	-	1056	WP_014529838.1	dipeptidase	-
JFX10_RS20315	2618101..2618442	-	342	WP_003536339.1	EamA family transporter	-
JFX10_RS20320	2618490..2619050	-	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
JFX10_RS20325	2619187..2619459	+	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JFX10_RS20330	2619456..2619860	+	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX10_RS20335	2619988..2620380	+	393	WP_003536349.1	globin	-
JFX10_RS20340	2620386..2620760	+	375	WP_010969927.1	DUF423 domain-containing protein	-
JFX10_RS20345	2620883..2621083	+	201	WP_042709455.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JFX10_RS20350	2621257..2621571	-	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
JFX10_RS20355	2621584..2621850	-	267	WP_010969929.1	hypothetical protein	-
JFX10_RS20360	2621903..2622316	-	414	WP_003536357.1	DUF2325 domain-containing protein	-
JFX10_RS20365	2622524..2623450	-	927	WP_010969930.1	energy transducer TonB	-
JFX10_RS20370	2623491..2623925	-	435	WP_010969931.1	hypothetical protein	-
JFX10_RS20375	2624155..2624295	+	141	WP_010969932.1	hemin uptake protein HemP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T184323 WP_010969926.1 NZ_CP066359:2619456-2619860 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T184323 NZ_CP087613:c6201-5896 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT184323 WP_003536345.1 NZ_CP066359:2619187-2619459 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT184323 NZ_CP087613:c6421-6203 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10