Detailed information of TA system TA184322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2212275..2212929
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: JFX10_RS18410
• Protein ID: WP_003530010.1
• Coordinates: 2212275..2212463 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: JFX10_RS18415
• Protein ID: WP_010969659.1
• Coordinates: 2212468..2212929 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS18395	2208841..2210151	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
JFX10_RS18400	2210176..2211147	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
JFX10_RS18405	2211328..2212098	-	771	WP_010969658.1	hypothetical protein	-
JFX10_RS18410	2212275..2212463	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
JFX10_RS18415	2212468..2212929	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JFX10_RS18425	2213248..2214165	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
JFX10_RS18430	2214342..2215091	-	750	WP_010969660.1	NUDIX hydrolase	-
JFX10_RS18435	2215094..2216200	-	1107	WP_003530001.1	endonuclease	-
JFX10_RS18440	2216351..2216794	-	444	WP_003529999.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T184322 WP_003530010.1 NZ_CP066359:2212275-2212463 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T184322 NZ_CP087612:211537-211821 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT184322 WP_010969659.1 NZ_CP066359:2212468-2212929 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT184322 NZ_CP087612:211299-211547 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0