184321

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 1364627..1365201 Replicon chromosome
Accession NZ_CP066359
Organism Sinorhizobium meliloti strain AK76

Toxin (Protein)


Gene name vapC Uniprot ID Q92QN4
Locus tag JFX10_RS14190 Protein ID WP_010969154.1
Coordinates 1364627..1364992 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92QN3
Locus tag JFX10_RS14195 Protein ID WP_010969155.1
Coordinates 1364986..1365201 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX10_RS14165 1359767..1361209 + 1443 WP_010969150.1 NADH-quinone oxidoreductase subunit NuoN -
JFX10_RS14170 1361206..1361973 + 768 WP_003531861.1 biotin--[acetyl-CoA-carboxylase] ligase -
JFX10_RS14175 1362001..1363668 + 1668 WP_010969151.1 ribonuclease J -
JFX10_RS14180 1363727..1364131 + 405 WP_010969152.1 methylmalonyl-CoA epimerase -
JFX10_RS14185 1364271..1364540 + 270 WP_014526683.1 DUF1467 family protein -
JFX10_RS14190 1364627..1364992 - 366 WP_010969154.1 type II toxin-antitoxin system VapC family toxin Toxin
JFX10_RS14195 1364986..1365201 - 216 WP_010969155.1 ribbon-helix-helix protein, CopG family Antitoxin
JFX10_RS14200 1365269..1365496 - 228 WP_017264047.1 hypothetical protein -
JFX10_RS14205 1365912..1367240 + 1329 WP_003531873.1 proline--tRNA ligase -
JFX10_RS14210 1367261..1368571 + 1311 WP_014529722.1 lipoprotein-releasing ABC transporter permease subunit -
JFX10_RS14215 1368589..1369275 + 687 WP_010969157.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13592.57 Da        Isoelectric Point: 5.1787

>T184321 WP_010969154.1 NZ_CP066359:c1364992-1364627 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Download         Length: 366 bp

>T184321 NZ_CP087612:c116815-116372 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8381.51 Da        Isoelectric Point: 8.6052

>AT184321 WP_010969155.1 NZ_CP066359:c1365201-1364986 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Download         Length: 216 bp

>AT184321 NZ_CP087612:c117042-116812 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92QN4

Antitoxin

Source ID Structure
AlphaFold DB Q92QN3

References