Detailed information of TA system TA184318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 637942..638519
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JFX10_RS10725
• Protein ID: WP_014528972.1
• Coordinates: 637942..638205 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: JFX10_RS10730
• Protein ID: WP_010968708.1
• Coordinates: 638202..638519 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS10685	633271..633711	+	441	WP_014528946.1	DUF2948 family protein	-
JFX10_RS10690	633775..635079	+	1305	WP_028004761.1	histidinol dehydrogenase	-
JFX10_RS10695	635076..635552	+	477	WP_010968704.1	UPF0262 family protein	-
JFX10_RS10700	635566..636045	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
JFX10_RS10705	636203..636421	+	219	WP_004435948.1	translation initiation factor IF-1	-
JFX10_RS10710	636520..637140	+	621	WP_014528949.1	Maf-like protein	-
JFX10_RS10715	637145..637357	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
JFX10_RS10725	637942..638205	+	264	WP_014528972.1	BrnT family toxin	Toxin
JFX10_RS10730	638202..638519	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
JFX10_RS10735	638722..639186	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
JFX10_RS10740	639183..639371	-	189	WP_017266533.1	hypothetical protein	-
JFX10_RS10745	639690..640790	+	1101	WP_017269979.1	HPr kinase	-
JFX10_RS10750	640777..641859	+	1083	WP_014528975.1	nucleotidyltransferase family protein	-
JFX10_RS10755	642062..642373	+	312	WP_014528976.1	hypothetical protein	-
JFX10_RS10760	642652..643041	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T184318 WP_014528972.1 NZ_CP066359:637942-638205 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASAIIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T184318 NZ_CP087611:c5242050-5241667 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT184318 WP_010968708.1 NZ_CP066359:638202-638519 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT184318 NZ_CP087611:c5242292-5242050 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: Q92KN5